Ser285
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Home > Phosphorylation Site Page: > Ser285  -  F3 (human)

Site Information
RKAGVGQsWkENsPL   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 451611

In vivo Characterization
Methods used to characterize site in vivo:
immunoassay ( 1 ) , immunoprecipitation ( 2 , 3 ) , mutation of modification site ( 3 , 5 , 7 , 8 , 10 ) , phospho-antibody ( 1 , 4 , 5 , 9 ) , western blotting ( 2 , 3 , 4 , 5 )
Disease tissue studied:
breast cancer ( 2 , 3 ) , colorectal cancer ( 7 ) , colorectal carcinoma ( 7 )
Relevant cell line - cell type - tissue:
CHO (fibroblast) [EphB1 (human), transfection] ( 9 ) , E.coli (bacterial) ( 2 ) , HAEC (endothelial) ( 3 , 4 ) , HCAEC (endothelial) ( 5 ) , HDMEC (endothelial) ( 5 ) , HT-29 (intestinal) ( 7 ) , HUVEC (endothelial) ( 5 , 9 , 10 ) , MDA-MB-231 (breast cell) ( 2 , 3 , 9 ) , PAE (endothelial) [PDGFRB (human)] ( 8 ) , urine ( 1 )

Upstream Regulation
Putative in vivo kinases:
PKCA (human) ( 9 )
Kinases, in vitro:
P38A (human) ( 4 )
Phosphatases, in vitro:
PPP2CA (human) ( 3 )
Treatments:
calyculin_A ( 5 ) , Go_6976 ( 9 ) , Go_6983 ( 9 ) , hispidin ( 9 ) , PAR2-activating_peptide ( 2 ) , peptide_agonist ( 5 ) , safingol ( 9 ) , thrombin ( 9 )

Downstream Regulation
Effects of modification on F3:
intracellular localization ( 5 ) , molecular association, regulation ( 2 ) , phosphorylation ( 3 , 5 , 6 , 9 , 10 ) , protein conformation ( 6 ) , ubiquitination ( 3 )
Effects of modification on biological processes:
apoptosis, altered ( 5 ) , cell growth, altered ( 7 ) , cell motility, altered ( 8 )
Induce interaction with:
FLNA (human) ( 2 )

References 

1

Greenfield RS, et al. (2018) Signal Transduction Peptide of Tissue Factor Phosphorylated at Ser258 and the Unphosphorylated STP in Urine Are Potential Biomarkers for Bladder Cancer. Clin Genitourin Cancer
30579699   Curated Info

2

Collier MEW, et al. (2017) Investigation of the Filamin A-Dependent Mechanisms of Tissue Factor Incorporation into Microvesicles. Thromb Haemost 117, 2034-2044
29044292   Curated Info

3

Ettelaie C, et al. (2016) Oligoubiquitination of tissue factor on Lys255 promotes Ser253-dephosphorylation and terminates TF release. Biochim Biophys Acta 1863, 2846-2857
27599717   Curated Info

4

Ettelaie C, Elkeeb AM, Maraveyas A, Collier ME (2013) p38α phosphorylates serine 258 within the cytoplasmic domain of tissue factor and prevents its incorporation into cell-derived microparticles. Biochim Biophys Acta 1833, 613-21
23195225   Curated Info

5

Collier ME, Ettelaie C (2011) Regulation of the incorporation of tissue factor into microparticles by serine phosphorylation of the cytoplasmic domain of tissue factor. J Biol Chem 286, 11977-84
21310953   Curated Info

6

Sen M, et al. (2009) Spectroscopic Characterization of Successive Phosphorylation of the Tissue Factor Cytoplasmic Region. Open Spectrosc J 3, 58-64
20076769   Curated Info

7

Li C, et al. (2008) Investigation of the mechanisms of tissue factor-mediated evasion of tumour cells from cellular cytotoxicity. Cancer Immunol Immunother 57, 1347-55
18297283   Curated Info

8

Siegbahn A, et al. (2005) Regulation of chemotaxis by the cytoplasmic domain of tissue factor. Thromb Haemost 93, 27-34
15630487   Curated Info

9

Ahamed J, Ruf W (2004) Protease-activated receptor 2-dependent phosphorylation of the tissue factor cytoplasmic domain. J Biol Chem 279, 23038-44
15039423   Curated Info

10

Dorfleutner A, Ruf W (2003) Regulation of tissue factor cytoplasmic domain phosphorylation by palmitoylation. Blood 102, 3998-4005
12920028   Curated Info

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