Ser812
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Home > Phosphorylation Site Page: > Ser812  -  PKD2 (human)

Site Information
FPRSLDDsEEDDDED   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 451598

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 17 ) , immunoassay ( 9 ) , immunoprecipitation ( 17 ) , mass spectrometry ( 1 , 2 , 3 , 4 , 5 , 7 , 10 , 12 , 13 , 14 , 15 , 16 ) , mutation of modification site ( 6 , 9 , 17 ) , phospho-antibody ( 9 ) , western blotting ( 9 )
Disease tissue studied:
breast cancer ( 3 ) , breast ductal carcinoma ( 3 ) , lung cancer ( 5 , 13 ) , non-small cell lung cancer ( 13 ) , non-small cell lung adenocarcinoma ( 5 ) , ovarian cancer ( 3 ) , melanoma skin cancer ( 2 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 9 , 12 ) , A549 (pulmonary) ( 7 ) , breast ( 3 ) , E.coli (bacterial) ( 6 ) , endothelial-aorta ( 10 ) , HeLa (cervical) ( 15 , 16 ) , HUES-9 ('stem, embryonic') ( 14 ) , liver ( 4 ) , LLC-PK1 (renal) [PKD2 (human)] ( 17 ) , lung ( 5 ) , NCI-H1650 (pulmonary) ( 13 ) , ovary ( 3 ) , Vero E6-S ('epithelial, kidney') ( 1 ) , WM239A (melanocyte) ( 2 )

Upstream Regulation
Kinases, in vitro:
CK2A1 (human) ( 17 )

Downstream Regulation
Effects of modification on PKD2:
activity, induced ( 17 ) , molecular association, regulation ( 6 )
Induce interaction with:
PACS2 (human) ( 6 )
Inhibit interaction with:
CBY1 (human) ( 6 )

References 

1

Bouhaddou M, et al. (2020) The Global Phosphorylation Landscape of SARS-CoV-2 Infection. Cell
32645325   Curated Info

2

Stuart SA, et al. (2015) A Phosphoproteomic Comparison of B-RAFV600E and MKK1/2 Inhibitors in Melanoma Cells. Mol Cell Proteomics 14, 1599-615
25850435   Curated Info

3

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

4

Bian Y, et al. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics 96, 253-62
24275569   Curated Info

5

Schweppe DK, Rigas JR, Gerber SA (2013) Quantitative phosphoproteomic profiling of human non-small cell lung cancer tumors. J Proteomics 91, 286-96
23911959   Curated Info

6

Morick D, et al. (2013) Phosphorylation of C-terminal polycystin-2 influences the interaction with PIGEA14: A QCM study based on solid supported membranes. Biochem Biophys Res Commun 437, 532-7
23838289   Curated Info

7

Kim JY, et al. (2013) Dissection of TBK1 signaling via phosphoproteomics in lung cancer cells. Proc Natl Acad Sci U S A 110, 12414-9
23836654   Curated Info

8

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

9

Streets AJ, Wessely O, Peters DJ, Ong AC (2013) Hyperphosphorylation of polycystin-2 at a critical residue in disease reveals an essential role for polycystin-1-regulated dephosphorylation. Hum Mol Genet 22, 1924-39
23390129   Curated Info

10

Verano-Braga T, et al. (2012) Time-resolved quantitative phosphoproteomics: new insights into Angiotensin-(1-7) signaling networks in human endothelial cells. J Proteome Res 11, 3370-81
22497526   Curated Info

11

Beli P, et al. (2012) Proteomic Investigations Reveal a Role for RNA Processing Factor THRAP3 in the DNA Damage Response. Mol Cell 46, 212-25
22424773   Curated Info

12

Mulhern D (2011) CST Curation Set: 12826; Year: 2011; Biosample/Treatment: cell line, HEK 293/WYE-354; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) AMPK Substrate (P-S/T(2)-102) Rabbit mAb Cat#: 5759, PTMScan(R) Phospho-AMPK Substrate Motif (LXRXXS*/T*) Immunoaffinity Beads Cat#: 5760
Curated Info

13

Possemato A (2011) CST Curation Set: 11906; Year: 2011; Biosample/Treatment: cell line, NCI-H1650/untreated; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

14

Rigbolt KT, et al. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal 4, rs3
21406692   Curated Info

15

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info

16

Pan C, Olsen JV, Daub H, Mann M (2009) Global effects of kinase inhibitors on signaling networks revealed by quantitative phosphoproteomics. Mol Cell Proteomics 8, 2796-808
19651622   Curated Info

17

Cai Y, et al. (2004) Calcium dependence of polycystin-2 channel activity is modulated by phosphorylation at Ser812. J Biol Chem 279, 19987-95
14742446   Curated Info