Ser549

Site Information
PAARPPAsPsPQRQA SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 451574

In vivo Characterization
Methods used to characterize site in vivo:	[32P] bio-synthetic labeling ( 6 ) , mass spectrometry ( 2 , 3 ) , phospho-antibody ( 4 , 5 ) , phosphopeptide mapping ( 6 ) , western blotting ( 4 , 5 )
Relevant cell line - cell type - tissue:	'brain, brainstem' ( 3 ) , 'brain, cerebellum' ( 3 ) , 'brain, hypothalamus' ( 4 ) , 'neuron, cortical'-brain ( 6 ) , PC-12 (chromaffin) [TrkA (rat), transfection] ( 2 ) , PC-12 (chromaffin) ( 6 ) , SF9 ( 5 )

Upstream Regulation
Putative in vivo kinases:	ERK1 (rat) ( 6 ) , ERK2 (rat) ( 6 )
Kinases, in vitro:	ERK1 (human) ( 6 )
Treatments:	BDNF ( 6 ) , depolarization ( 6 ) , estradiol ( 4 ) , NGF ( 6 ) , okadaic_acid ( 5 )

References
1	Verstegen AM, et al. (2014) Phosphorylation of Synapsin I by Cyclin-Dependent Kinase-5 Sets the Ratio between the Resting and Recycling Pools of Synaptic Vesicles at Hippocampal Synapses. J Neurosci 34, 7266-80 24849359 Curated Info
2	Biarc J, Chalkley RJ, Burlingame AL, Bradshaw RA (2012) The induction of serine/threonine protein phosphorylations by a PDGFR/TrkA chimera in stably transfected PC12 cells. Mol Cell Proteomics 11, 15-30 22027198 Curated Info
3	Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876 22673903 Curated Info
4	Ohtani-Kaneko R, et al. (2010) Effects of estrogen on synapsin I distribution in developing hypothalamic neurons. Neurosci Res 66, 180-8 19896987 Curated Info
5	Tu Y, Nayak SK, Woodson J, Ross EM (2003) Phosphorylation-regulated inhibition of the Gz GTPase-activating protein activity of RGS proteins by synapsin I. J Biol Chem 278, 52273-81 14557263 Curated Info
6	Jovanovic JN, et al. (1996) Neurotrophins stimulate phosphorylation of synapsin I by MAP kinase and regulate synapsin I-actin interactions. Proc Natl Acad Sci U S A 93, 3679-83 8622996 Curated Info