Thr253
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Home > Phosphorylation Site Page: > Thr253  -  CAMK2A (rat)

Site Information
DLINkMLtINPskRI   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 451571

In vivo Characterization
Methods used to characterize site in vivo:
flow cytometry ( 1 ) , mass spectrometry ( 4 ) , mass spectrometry (in vitro) ( 2 ) , mutation of modification site ( 1 ) , phospho-antibody ( 1 , 3 , 5 ) , western blotting ( 1 )
Disease tissue studied:
breast cancer ( 1 ) , neuroblastoma ( 1 )
Relevant cell line - cell type - tissue:
'brain, cerebellum' ( 5 ) , 'brain, cerebral cortex' ( 5 ) , 'brain, hippocampus' ( 3 , 5 ) , brain ( 4 ) , MDA-MB-231 (breast cell) ( 1 ) , SH-SY5Y (neural crest) ( 1 )

Upstream Regulation
Kinases, in vitro:
CAMK2A (rat) ( 2 , 5 , 6 , 7 ) , CAMK2B (rat) ( 2 )
Putative upstream phosphatases:
PPP2CA (human) ( 1 )
Treatments:
depolarization ( 5 ) , ischemia/reperfusion ( 3 ) , status epilepticus ( 3 )

Downstream Regulation
Effects of modification on CAMK2A:
intracellular localization ( 5 )
Effects of modification on biological processes:
apoptosis, induced ( 1 ) , cell cycle regulation ( 1 ) , cell growth, inhibited ( 1 )

References 

1

Hoffman A, et al. (2014) Dephosphorylation of CaMKII at T253 controls the metaphase-anaphase transition. Cell Signal 26, 748-56
24407174   Curated Info

2

Dosemeci A, Jaffe H (2010) Regulation of phosphorylation at the postsynaptic density during different activity states of Ca2+/calmodulin-dependent protein kinase II. Biochem Biophys Res Commun 391, 78-84
19896464   Curated Info

3

Gurd JW, et al. (2008) Ischemia and status epilepitcus result in enhanced phosphorylation of calcium and calmodulin-stimulated protein kinase II on threonine 253. Brain Res 1218, 158-65
18514171   Curated Info

4

Guo A (2007) CST Curation Set: 2239; Year: 2007; Biosample/Treatment: tissue, brain/ischemia 20'; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]Q Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) ATM/ATR Substrate Antibody Cat#: 2851
Curated Info

5

Migues PV, et al. (2006) Phosphorylation of CaMKII at Thr253 occurs in vivo and enhances binding to isolated postsynaptic densities. J Neurochem 98, 289-99
16805815   Curated Info

6

Lengyel I, et al. (2000) Modulation of the phosphorylation and activity of calcium/calmodulin-dependent protein kinase II by zinc. J Neurochem 75, 594-605
10899934   Curated Info

7

Dosemeci A, Gollop N, Jaffe H (1994) Identification of a major autophosphorylation site on postsynaptic density-associated Ca2+/calmodulin-dependent protein kinase. J Biol Chem 269, 31330-3
7989295   Curated Info