Ser303

Site Information
sAPPPKKsLGTQPPK SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 13357130

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 2 , 3 , 5 , 6 , 7 , 8 , 9 )
Disease tissue studied:	HER2 positive breast cancer ( 1 ) , luminal A breast cancer ( 1 ) , luminal B breast cancer ( 1 ) , breast cancer, surrounding tissue ( 1 ) , breast cancer, triple negative ( 1 ) , cervical cancer ( 8 ) , cervical adenocarcinoma ( 8 ) , acute erythroid leukemias, including erythroleukemia (M6a) and very rare pure erythroid leukemia (M6b) ( 6 ) , acute megakaryoblastic leukemia (M7) ( 6 ) , acute monoblastic leukemia (M5a) or acute monocytic leukemia (M5b) ( 6 ) , acute myeloblastic leukemia, with granulocytic maturation (M2) ( 6 ) , acute myeloblastic leukemia, without maturation (M1) ( 6 ) , B cell lymphoma ( 6 ) , non-Hodgkin's lymphoma ( 6 ) , multiple myeloma ( 6 )
Relevant cell line - cell type - tissue:	AML-193 (monocyte) ( 6 ) , breast ( 1 ) , CMK (megakaryoblast) ( 6 ) , CTS (myeloid) ( 6 ) , DOHH2 ('B lymphocyte, precursor') ( 6 ) , HEL (erythroid) ( 6 ) , HeLa (cervical) ( 2 , 5 , 7 ) , HeLa S3 (cervical) ( 8 ) , Jurkat (T lymphocyte) ( 3 ) , Kasumi-1 (myeloid) ( 6 ) , KG-1 (myeloid) ( 6 ) , MCF10CA1a c11 (epithelial) ( 9 ) , MV4-11 (macrophage) ( 6 ) , OPM-2 (plasma cell) ( 6 ) , P31/FUJ (erythroid) ( 6 ) , RL ('B lymphocyte, precursor') ( 6 ) , RPMI-8266 (plasma cell) ( 6 ) , SU-DHL-6 (B lymphocyte) ( 6 ) , U266 (plasma cell) ( 6 )

Upstream Regulation
Treatments:	BI2536 ( 7 ) , nocodazole ( 8 )

References
1	Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62 27251275 Curated Info
2	Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94 25159151 Curated Info
3	Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7 23749302 Curated Info
4	Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21 23312004 Curated Info
5	Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71 23186163 Curated Info
6	Casado P, et al. (2013) Phosphoproteomics data classify hematological cancer cell lines according to tumor type and sensitivity to kinase inhibitors. Genome Biol 14, R37 23628362 Curated Info
7	Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5 21712546 Curated Info
8	Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3 20068231 Curated Info
9	Patwa TH, et al. (2008) A novel phosphoprotein analysis scheme for assessing changes in premalignant and malignant breast cell lines using 2D liquid separations, protein microarrays and tandem mass spectrometry. Proteomics Clin Appl 3, 51-66 19194518 Curated Info