Ser333
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Home > Phosphorylation Site Page: > Ser333  -  BCKDH E1-alpha (rat)

Site Information
TYRIGHHstsDDSSA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 449636

In vivo Characterization
Methods used to characterize site in vivo:
[32P] ATP in vitro ( 10 ) , [32P] bio-synthetic labeling ( 10 ) , mass spectrometry ( 2 , 3 , 4 , 5 , 6 , 7 ) , peptide sequencing ( 10 ) , phospho-antibody ( 1 , 8 ) , western blotting ( 1 )
Disease tissue studied:
type 2 diabetes ( 5 )
Relevant cell line - cell type - tissue:
'brain, brainstem' ( 3 ) , 'brain, cerebellum' ( 3 ) , 'brain, cerebral cortex' ( 3 ) , adipocyte-adipose tissue ( 8 ) , adipose tissue ( 3 ) , brain ( 1 ) , heart ( 3 , 6 , 10 ) , hepatocyte-liver ( 8 ) , IEC-6 (epithelial) ( 2 ) , intestine ( 3 ) , kidney ( 3 ) , liver ( 3 , 4 , 5 , 7 ) , lung ( 3 ) , muscle ( 3 ) , pancreas ( 3 ) , spleen ( 3 ) , stomach ( 3 ) , testis ( 3 ) , thymus ( 3 ) , TREMK4 ( 8 )

Upstream Regulation
Putative in vivo kinases:
BCKDK (rat) ( 1 , 8 )
Kinases, in vitro:
BCKDK (human) ( 10 ) , BCKDK (rat) ( 9 )
Treatments:
amino_acid_starvation ( 8 ) , amino_acids ( 8 ) , food deprivation ( 8 ) , serum ( 2 )

Downstream Regulation
Effects of modification on BCKDH E1-alpha:
enzymatic activity, inhibited ( 1 , 10 )
Effects of modification on biological processes:
cell growth, induced ( 1 )

References 

1

Zigler JS, et al. (2016) A Spontaneous Missense Mutation in Branched Chain Keto Acid Dehydrogenase Kinase in the Rat Affects Both the Central and Peripheral Nervous Systems. PLoS One 11, e0160447
27472223   Curated Info

2

Courcelles M, et al. (2013) Phosphoproteome dynamics reveal novel ERK1/2 MAP kinase substrates with broad spectrum of functions. Mol Syst Biol 9, 669
23712012   Curated Info

3

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

4

Demirkan G, et al. (2011) Phosphoproteomic Profiling of In Vivo Signaling in Liver by the Mammalian Target of Rapamycin Complex 1 (mTORC1). PLoS One 6, e21729
21738781   Curated Info

5

Deng WJ, et al. (2010) Proteome, phosphoproteome, and hydroxyproteome of liver mitochondria in diabetic rats at early pathogenic stages. Mol Cell Proteomics 9, 100-16
19700791   Curated Info

6

Feng J, et al. (2008) Phosphoproteome analysis of isoflurane-protected heart mitochondria: phosphorylation of adenine nucleotide translocator-1 on Tyr194 regulates mitochondrial function. Cardiovasc Res 80, 20-9
18558627   Curated Info

7

Moser K, White FM (2006) Phosphoproteomic analysis of rat liver by high capacity IMAC and LC-MS/MS. J Proteome Res 5, 98-104
16396499   Curated Info

8

Lynch CJ, et al. (2003) Potential role of leucine metabolism in the leucine-signaling pathway involving mTOR. Am J Physiol Endocrinol Metab 285, E854-63
12812918   Curated Info

9

Zhao Y, et al. (1994) Site-directed mutagenesis of phosphorylation sites of the branched chain alpha-ketoacid dehydrogenase complex. J Biol Chem 269, 18583-7
8034607   Curated Info

10

Paxton R, Kuntz M, Harris RA (1986) Phosphorylation sites and inactivation of branched-chain alpha-ketoacid dehydrogenase isolated from rat heart, bovine kidney, and rabbit liver, kidney, heart, brain, and skeletal muscle. Arch Biochem Biophys 244, 187-201
3947057   Curated Info