Ser535
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Home > Phosphorylation Site Page: > Ser535  -  NFkB-p65 (rat)

Site Information
sGDEDFSsIADMDFS   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447800

In vivo Characterization
Methods used to characterize site in vivo:
mutation of modification site ( 3 ) , phospho-antibody ( 3 , 4 , 5 , 6 , 7 ) , western blotting ( 3 , 4 , 5 , 6 , 7 )
Disease tissue studied:
colorectal cancer ( 6 , 7 ) , colorectal carcinoma ( 6 , 7 ) , liver cancer ( 3 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 5 ) , aorta ( 4 ) , CMT-93 (intestinal) ( 7 ) , HCT116 (intestinal) ( 6 ) , HepG2 (hepatic) ( 3 ) , HT-29 (intestinal) ( 6 ) , intestine ( 7 ) , liver ( 3 ) , vascular smooth muscle cell ('muscle, smooth') ( 4 , 5 )

Controlled by
Regulatory protein:
IKKB (rat) ( 5 ) , RHOA (human) ( 4 )
Putative upstream phosphatases:
PPP2CA (mouse) ( 7 )
Treatments:
5-ASA ( 6 ) , angiotensin ( 5 ) , angiotensin_2 ( 3 , 4 ) , exoenzyme_C3 ( 4 ) , glucosamine ( 2 ) , PD98059 ( 5 ) , PUGNAc ( 2 ) , siRNA ( 5 ) , status epilepticus ( 1 ) , taurine_chloramine ( 6 ) , TNF ( 2 , 6 ) , U0126 ( 5 )

References 

1

Ryu HJ, et al. (2011) ReLA/P65-serine 536 nuclear factor-kappa B phosphorylation is related to vulnerability to status epilepticus in the rat hippocampus. Neuroscience 187, 93-102
21550382   Curated Info

2

Xing D, et al. (2011) O-GlcNAc modification of NFκB p65 inhibits TNF-α-induced inflammatory mediator expression in rat aortic smooth muscle cells. PLoS One 6, e24021
21904602   Curated Info

3

Choudhary S, Lu M, Cui R, Brasier AR (2007) Involvement of a novel Rac/RhoA guanosine triphosphatase-nuclear factor-kappaB inducing kinase signaling pathway mediating angiotensin II-induced RelA transactivation. Mol Endocrinol 21, 2203-17
17595324   Curated Info

4

Cui R, et al. (2006) RhoA mediates angiotensin II-induced phospho-Ser536 nuclear factor kappaB/RelA subunit exchange on the interleukin-6 promoter in VSMCs. Circ Res 99, 723-30
16960103   Curated Info

5

Douillette A, et al. (2006) The proinflammatory actions of angiotensin II are dependent on p65 phosphorylation by the IkappaB kinase complex. J Biol Chem 281, 13275-84
16513650   Curated Info

6

Kim H, et al. (2006) A molecular mechanism for the anti-inflammatory effect of taurine-conjugated 5-aminosalicylic acid in inflamed colon. Mol Pharmacol 69, 1405-12
16407467   Curated Info

7

Ruiz PA, Kim SC, Sartor RB, Haller D (2004) 15-deoxy-delta12,14-prostaglandin J2-mediated ERK signaling inhibits gram-negative bacteria-induced RelA phosphorylation and interleukin-6 gene expression in intestinal epithelial cells through modulation of protein phosphatase 2A activity. J Biol Chem 279, 36103-11
15199053   Curated Info