Ser535
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Home > Phosphorylation Site Page: > Ser535  -  NFkB-p65 (rat)

Site Information
sGDEDFSsIADMDFS   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447800

In vivo Characterization
Methods used to characterize site in vivo:
immunoassay ( 2 ) , mutation of modification site ( 4 ) , phospho-antibody ( 2 , 3 , 4 , 5 , 6 , 7 , 8 ) , western blotting ( 3 , 4 , 5 , 6 , 7 , 8 )
Disease tissue studied:
colorectal cancer ( 7 , 8 ) , colorectal carcinoma ( 7 , 8 ) , liver cancer ( 4 )
Relevant cell line - cell type - tissue:
'epithelial, lung' ( 1 ) , 'muscle, smooth'-thoracic aorta ( 3 ) , 'neuron, hippocampal, CA1 pyramidal' ( 2 ) , 293 (epithelial) ( 6 ) , aorta ( 5 ) , CMT-93 (intestinal) ( 8 ) , HCT116 (intestinal) ( 7 ) , HepG2 (hepatic) ( 4 ) , HT-29 (intestinal) ( 7 ) , intestine ( 8 ) , liver ( 4 ) , vascular smooth muscle cell ('muscle, smooth') ( 5 , 6 )

Upstream Regulation
Regulatory protein:
IKKB (rat) ( 6 ) , RHOA (human) ( 5 )
Putative upstream phosphatases:
PPP2CA (mouse) ( 8 )
Treatments:
5-ASA ( 7 ) , angiotensin ( 6 ) , angiotensin_2 ( 4 , 5 ) , exoenzyme_C3 ( 5 ) , glucosamine ( 3 ) , PD98059 ( 6 ) , PUGNAc ( 3 ) , siRNA ( 6 ) , status epilepticus ( 2 ) , taurine_chloramine ( 7 ) , titanium dioxide nanoparticles ( 1 ) , TNF ( 3 , 7 ) , U0126 ( 6 )

Downstream Regulation
Effects of modification on biological processes:
apoptosis, induced ( 2 ) , apoptosis, inhibited ( 2 ) , signaling pathway regulation ( 3 )

References 

1

Abukabda AB, et al. (2018) Group II innate lymphoid cells and microvascular dysfunction from pulmonary titanium dioxide nanoparticle exposure. Part Fibre Toxicol 15, 43
30413212   Curated Info

2

Ryu HJ, et al. (2011) ReLA/P65-serine 536 nuclear factor-kappa B phosphorylation is related to vulnerability to status epilepticus in the rat hippocampus. Neuroscience 187, 93-102
21550382   Curated Info

3

Xing D, et al. (2011) O-GlcNAc modification of NFκB p65 inhibits TNF-α-induced inflammatory mediator expression in rat aortic smooth muscle cells. PLoS One 6, e24021
21904602   Curated Info

4

Choudhary S, Lu M, Cui R, Brasier AR (2007) Involvement of a novel Rac/RhoA guanosine triphosphatase-nuclear factor-kappaB inducing kinase signaling pathway mediating angiotensin II-induced RelA transactivation. Mol Endocrinol 21, 2203-17
17595324   Curated Info

5

Cui R, et al. (2006) RhoA mediates angiotensin II-induced phospho-Ser536 nuclear factor kappaB/RelA subunit exchange on the interleukin-6 promoter in VSMCs. Circ Res 99, 723-30
16960103   Curated Info

6

Douillette A, et al. (2006) The proinflammatory actions of angiotensin II are dependent on p65 phosphorylation by the IkappaB kinase complex. J Biol Chem 281, 13275-84
16513650   Curated Info

7

Kim H, et al. (2006) A molecular mechanism for the anti-inflammatory effect of taurine-conjugated 5-aminosalicylic acid in inflamed colon. Mol Pharmacol 69, 1405-12
16407467   Curated Info

8

Ruiz PA, Kim SC, Sartor RB, Haller D (2004) 15-deoxy-delta12,14-prostaglandin J2-mediated ERK signaling inhibits gram-negative bacteria-induced RelA phosphorylation and interleukin-6 gene expression in intestinal epithelial cells through modulation of protein phosphatase 2A activity. J Biol Chem 279, 36103-11
15199053   Curated Info