Ser379
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.6.0.2
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser379  -  p47phox (human)

Site Information
DLILNRCsEstKRKL   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 451498

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 10 , 11 ) , immunoprecipitation ( 4 , 5 ) , mass spectrometry ( 2 ) , mutation of modification site ( 3 , 4 , 5 , 6 , 9 , 10 ) , peptide sequencing ( 11 ) , phospho-antibody ( 5 ) , phosphoamino acid analysis ( 11 ) , phosphopeptide mapping ( 11 ) , western blotting ( 5 )
Disease tissue studied:
HER2 positive breast cancer ( 2 ) , luminal A breast cancer ( 2 ) , luminal B breast cancer ( 2 ) , breast cancer, triple negative ( 2 ) , leukemia ( 9 ) , chronic myelogenous leukemia ( 9 )
Relevant cell line - cell type - tissue:
B lymphocyte-spleen ( 10 ) , breast ( 2 ) , COS7 (fibroblast) ( 5 ) , K562 (erythroid) ( 6 , 9 ) , microvessel endothelial ( 3 ) , neutrophil ( 11 ) , Rat2 (fibroblast) ( 4 ) , SVEC (endothelial) ( 5 )

Upstream Regulation
Putative in vivo kinases:
PKCD (human) ( 4 )
Kinases, in vitro:
PKCA (human) ( 8 ) , PKCB iso2 (human) ( 8 ) , PKCD (human) ( 4 , 8 )
Treatments:
phorbol_ester ( 3 , 10 )

Downstream Regulation
Effects of modification on p47phox:
enzymatic activity, induced ( 4 ) , intracellular localization ( 3 , 4 , 5 , 10 ) , molecular association, regulation ( 3 , 5 , 6 , 7 , 8 ) , phosphorylation ( 5 ) , protein conformation ( 3 )
Effects of modification on biological processes:
carcinogenesis, induced ( 4 ) , cell growth, induced ( 4 ) , signaling pathway regulation ( 5 )
Induce interaction with:
CYBA (human) ( 3 , 8 ) , TRAF4 (human) ( 5 )
Inhibit interaction with:
p40phox (human) ( 7 ) , p67phox (human) ( 6 , 7 )

References 

1

Neehus AL, et al. (2021) Impaired respiratory burst contributes to infections in PKCδ-deficient patients. J Exp Med 218
34264265   Curated Info

2

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

3

Meijles DN, Fan LM, Howlin BJ, Li JM (2014) Molecular Insights of p47phox Phosphorylation Dynamics in the Regulation of NADPH Oxidase Activation and Superoxide Production. J Biol Chem 289, 22759-70
24970888   Curated Info

4

Park MT, et al. (2014) Novel signaling axis for ROS generation during K-Ras-induced cellular transformation. Cell Death Differ 21, 1185-97
24632950   Curated Info

5

Teng L, Fan LM, Meijles D, Li JM (2012) Divergent Effects of p47phox Phosphorylation at S303-4 or S379 on Tumor Necrosis Factor-α Signaling via TRAF4 and MAPK in Endothelial Cells. Arterioscler Thromb Vasc Biol 32, 1488-96
22460559   Curated Info

6

Mizuki K, et al. (2005) A region C-terminal to the proline-rich core of p47phox regulates activation of the phagocyte NADPH oxidase by interacting with the C-terminal SH3 domain of p67phox. Arch Biochem Biophys 444, 185-94
16297854   Curated Info

7

Massenet C, et al. (2005) Effects of p47phox C terminus phosphorylations on binding interactions with p40phox and p67phox. Structural and functional comparison of p40phox and p67phox SH3 domains. J Biol Chem 280, 13752-61
15657040   Curated Info

8

Fontayne A, Dang PM, Gougerot-Pocidalo MA, El-Benna J (2002) Phosphorylation of p47phox sites by PKC alpha, beta II, delta, and zeta: effect on binding to p22phox and on NADPH oxidase activation. Biochemistry 41, 7743-50
12056906   Curated Info

9

Ago T, Nunoi H, Ito T, Sumimoto H (1999) Mechanism for phosphorylation-induced activation of the phagocyte NADPH oxidase protein p47(phox). Triple replacement of serines 303, 304, and 328 with aspartates disrupts the SH3 domain-mediated intramolecular interaction in p47(phox), thereby activating the oxidase. J Biol Chem 274, 33644-53
10559253   Curated Info

10

Faust LR, el Benna J, Babior BM, Chanock SJ (1995) The phosphorylation targets of p47phox, a subunit of the respiratory burst oxidase. Functions of the individual target serines as evaluated by site-directed mutagenesis. J Clin Invest 96, 1499-505
7657821   Curated Info

11

el Benna J, Faust LP, Babior BM (1994) The phosphorylation of the respiratory burst oxidase component p47phox during neutrophil activation. Phosphorylation of sites recognized by protein kinase C and by proline-directed kinases. J Biol Chem 269, 23431-6
8089108   Curated Info