Thr596

Site Information
ETLPISStPSKSVLP SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 451437

In vivo Characterization
Methods used to characterize site in vivo:	[32P] bio-synthetic labeling ( 4 ) , electrophoretic mobility shift ( 4 ) , mass spectrometry ( 1 , 2 , 3 ) , mutation of modification site ( 1 , 4 ) , western blotting ( 1 )
Disease tissue studied:	bone cancer ( 1 , 4 )
Relevant cell line - cell type - tissue:	HeLa (cervical) ( 2 , 3 ) , U2OS (bone cell) [PLK1 (human), transfection] ( 1 ) , U2OS (bone cell) ( 4 )

Downstream Regulation
Effects of modification on FOXM1 iso2:	molecular association, regulation ( 4 )
Effects of modification on biological processes:	transcription, induced ( 4 )
Induce interaction with:	CBP (human) ( 4 )

References
1	Chen YJ, et al. (2009) A conserved phosphorylation site within the forkhead domain of FoxM1B is required for its activation by cyclin-CDK1. J Biol Chem 284, 30695-707 19737929 Curated Info
2	Moritz A (2008) CST Curation Set: 5061; Year: 2008; Biosample/Treatment: cell line, HeLa/nocodazole; Disease: cervical adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P , PTMScan(R) PLK Binding Motif (SpTP) Immunoaffinity Beads Cat#: 1995 Curated Info
3	Moritz A (2008) CST Curation Set: 5070; Year: 2008; Biosample/Treatment: cell line, HeLa/nocodazole; Disease: cervical adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P , PTMScan(R) PLK Binding Motif (SpTP) Immunoaffinity Beads Cat#: 1995 Curated Info
4	Major ML, Lepe R, Costa RH (2004) Forkhead box M1B transcriptional activity requires binding of Cdk-cyclin complexes for phosphorylation-dependent recruitment of p300/CBP coactivators. Mol Cell Biol 24, 2649-61 15024056 Curated Info