Ser148
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.1
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser148  -  CD3G (human)

Site Information
GVRQsRAsDkQTLLP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 451430

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 5 , 6 , 8 ) , immunoprecipitation ( 5 ) , mass spectrometry ( 1 , 2 , 3 , 4 ) , mutation of modification site ( 5 , 6 )
Relevant cell line - cell type - tissue:
Jurkat (T lymphocyte) ( 1 , 2 , 3 , 4 , 5 , 6 )

Upstream Regulation
Putative in vivo kinases:
PKCA (human) ( 6 )
Kinases, in vitro:
PKCA (cow) ( 8 )
Treatments:
phorbol_ester ( 5 , 6 , 8 ) , phytohemagglutinin ( 8 )

Downstream Regulation
Effects of modification on CD3G:
activity, inhibited ( 5 ) , receptor internalization, altered ( 5 , 6 )

References 

1

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

2

Mulhern D (2011) CST Curation Set: 13111; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY]
Curated Info

3

Zhou J (2011) CST Curation Set: 12495; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

4

Zhou J (2011) CST Curation Set: 12496; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

5

Dietrich J, et al. (1996) Molecular characterization of the di-leucine-based internalization motif of the T cell receptor. J Biol Chem 271, 11441-8
8626701   Curated Info

6

Dietrich J, Hou X, Wegener AM, Geisler C (1994) CD3 gamma contains a phosphoserine-dependent di-leucine motif involved in down-regulation of the T cell receptor. EMBO J 13, 2156-66
8187769   Curated Info

7

Alexander D, et al. (1989) Dephosphorylation of the human T lymphocyte CD3 antigen. Eur J Biochem 181, 55-65
2540970   Curated Info

8

Davies AA, et al. (1987) The human T3 gamma chain is phosphorylated at serine 126 in response to T lymphocyte activation. J Biol Chem 262, 10918-21
3112151   Curated Info