Lys8
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Home > Ubiquitylation Site Page: > Lys8  -  Akt1 (human)

Site Information
MsDVAIVkEGWLHkR   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 12723503

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 4 ) , mass spectrometry ( 1 , 2 , 3 ) , mutation of modification site ( 4 ) , western blotting ( 4 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Treatments:
IGF-1 ( 4 ) , serum ( 4 )

Downstream Regulation
Effects of modification on Akt1:
enzymatic activity, induced ( 4 ) , intracellular localization ( 4 ) , phosphorylation ( 4 )

References 

1

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

2

Udeshi ND, et al. (2013) Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments. Mol Cell Proteomics 12, 825-31
23266961   Curated Info

3

Wagner SA, et al. (2011) A Proteome-wide, Quantitative Survey of In Vivo Ubiquitylation Sites Reveals Widespread Regulatory Roles. Mol Cell Proteomics 10, M111.013284
21890473   Curated Info

4

Yang WL, et al. (2009) The E3 ligase TRAF6 regulates Akt ubiquitination and activation. Science 325, 1134-8
19713527   Curated Info