Ser315

Site Information
GRMLQAIsPKQsPss SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 451321

In vivo Characterization
Methods used to characterize site in vivo:	[32P] bio-synthetic labeling ( 6 , 7 ) , mass spectrometry ( 1 , 2 , 3 , 4 , 5 ) , mutation of modification site ( 6 ) , phospho-antibody ( 6 ) , phosphoamino acid analysis ( 7 ) , phosphopeptide mapping ( 7 )
Relevant cell line - cell type - tissue:	'brain, brainstem' ( 3 ) , 'brain, cerebellum' ( 3 ) , 'brain, cerebral cortex' ( 3 ) , adipose tissue ( 3 ) , CHO (fibroblast) [EphB1 (human), transfection] ( 7 ) , heart ( 3 ) , IEC-6 (epithelial) ( 1 ) , intestine ( 3 ) , kidney ( 3 ) , liver ( 3 , 4 , 5 ) , lung ( 3 ) , MLE (epithelial) ( 6 ) , muscle ( 3 ) , pancreas ( 3 ) , PC-12 (chromaffin) [TrkA (rat), transfection] ( 2 ) , spleen ( 3 ) , stomach ( 3 ) , testis ( 3 ) , thymus ( 3 )

In vivo Characterization

Methods used to characterize site in vivo:

[32P] bio-synthetic labeling ( 6 , 7 ) , mass spectrometry ( 1 , 2 , 3 , 4 , 5 ) , mutation of modification site ( 6 ) , phospho-antibody ( 6 ) , phosphoamino acid analysis ( 7 ) , phosphopeptide mapping ( 7 )

Relevant cell line - cell type - tissue:

'brain, brainstem' ( 3 ) , 'brain, cerebellum' ( 3 ) , 'brain, cerebral cortex' ( 3 ) , adipose tissue ( 3 ) , CHO (fibroblast) [EphB1 (human), transfection] ( 7 ) , heart ( 3 ) , IEC-6 (epithelial) ( 1 ) , intestine ( 3 ) , kidney ( 3 ) , liver ( 3 , 4 , 5 ) , lung ( 3 ) , MLE (epithelial) ( 6 ) , muscle ( 3 ) , pancreas ( 3 ) , PC-12 (chromaffin) [TrkA (rat), transfection] ( 2 ) , spleen ( 3 ) , stomach ( 3 ) , testis ( 3 ) , thymus ( 3 )

Upstream Regulation
Putative in vivo kinases:	ERK1 (mouse) ( 6 ) , ERK2 (mouse) ( 6 )
Treatments:	22-hydroxycholesterol ( 6 ) , PD98059 ( 6 ) , retinoic_acid ( 6 ) , serum ( 1 )

Downstream Regulation
Effects of modification on PCYT1A:	enzymatic activity, inhibited ( 6 )

References
1	Courcelles M, et al. (2013) Phosphoproteome dynamics reveal novel ERK1/2 MAP kinase substrates with broad spectrum of functions. Mol Syst Biol 9, 669 23712012 Curated Info
2	Biarc J, Chalkley RJ, Burlingame AL, Bradshaw RA (2012) The induction of serine/threonine protein phosphorylations by a PDGFR/TrkA chimera in stably transfected PC12 cells. Mol Cell Proteomics 11, 15-30 22027198 Curated Info
3	Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876 22673903 Curated Info
4	Demirkan G, et al. (2011) Phosphoproteomic Profiling of In Vivo Signaling in Liver by the Mammalian Target of Rapamycin Complex 1 (mTORC1). PLoS One 6, e21729 21738781 Curated Info
5	Moser K, White FM (2006) Phosphoproteomic analysis of rat liver by high capacity IMAC and LC-MS/MS. J Proteome Res 5, 98-104 16396499 Curated Info
6	Agassandian M, et al. (2005) Oxysterols inhibit phosphatidylcholine synthesis via ERK docking and phosphorylation of CTP:phosphocholine cytidylyltransferase. J Biol Chem 280, 21577-87 15788406 Curated Info
7	MacDonald JI, Kent C (1994) Identification of phosphorylation sites in rat liver CTP: phosphocholine cytidylyltransferase. J Biol Chem 269, 10529-37 8144639 Curated Info