Ser27
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Home > Phosphorylation Site Page: > Ser27  -  G-alpha(z) (human)

Site Information
DRHLRSEsQRQRREI   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 451241

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 4 , 6 ) , mass spectrometry ( 1 , 2 ) , mutation of modification site ( 3 , 4 , 5 , 6 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 3 , 4 , 5 , 6 ) , Jurkat (T lymphocyte) ( 1 , 2 )

Upstream Regulation
Putative in vivo kinases:
PKCA (human) ( 6 )
Kinases, in vitro:
PKCA (human) ( 4 )
Treatments:
phorbol_ester ( 4 , 6 )

Downstream Regulation
Effects of modification on G-alpha(z):
activity, induced ( 3 , 5 ) , protein conformation ( 5 )

References 

1

Zhou J (2009) CST Curation Set: 5896; Year: 2009; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: (K/R)XpSX(K/R)Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser) PKC Substrate Antibody Cat#: 2261, PTMScan(R) Phospho-PKC Substrate Motif (K/RXpSXK/R) Immunoaffinity Beads Cat#: 1985
Curated Info

2

Zhou J (2009) CST Curation Set: 5897; Year: 2009; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: (K/R)XpSX(K/R)Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser) PKC Substrate Antibody Cat#: 2261, PTMScan(R) Phospho-PKC Substrate Motif (K/RXpSXK/R) Immunoaffinity Beads Cat#: 1985
Curated Info

3

Ho MK, Chan JS, Yung LY, Wong YH (2000) The effect of protein kinase C activation on G(z)-mediated regulation of type 2 and 6 adenylyl cyclases. Biol Signals Recept 9, 21-8
10686433   Curated Info

4

Wang J, Frost JA, Cobb MH, Ross EM (1999) Reciprocal signaling between heterotrimeric G proteins and the p21-stimulated protein kinase. J Biol Chem 274, 31641-7
10531372   Curated Info

5

Ho MK, Wong YH (1997) Functional role of amino-terminal serine16 and serine27 of G alphaZ in receptor and effector coupling. J Neurochem 68, 2514-22
9166747   Curated Info

6

Lounsbury KM, et al. (1993) Analysis of Gz alpha by site-directed mutagenesis. Sites and specificity of protein kinase C-dependent phosphorylation. J Biol Chem 268, 3494-8
8429024   Curated Info

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