Ser1179
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Home > Phosphorylation Site Page: > Ser1179  -  eNOS (pig)

Site Information
TSRIRtQsFSLQERH   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447884

In vivo Characterization
Methods used to characterize site in vivo:
mutation of modification site ( 2 ) , phospho-antibody ( 3 , 4 , 5 , 6 , 7 ) , western blotting ( 3 , 4 , 5 , 6 , 7 )
Relevant cell line - cell type - tissue:
artery-heart ( 3 ) , carotid artery [eNOS (pig), homozygous knockout] ( 2 ) , endothelial-aorta ( 5 ) , endothelial-pulmonary artery ( 6 ) , PAE (endothelial) ( 4 , 7 )

Upstream Regulation
Putative in vivo kinases:
Akt1 (human) ( 5 , 7 )
Treatments:
ACh ( 2 ) , AG1478 ( 7 ) , angiopoietin-1 ( 6 ) , BAPTA-AM ( 7 ) , black_tea_polyphenols ( 5 ) , bradykinin ( 3 ) , catalase ( 7 ) , faslodex ( 3 ) , geldanamycin ( 6 ) , H2O2 ( 7 ) , L-NAME ( 7 ) , LY294002 ( 4 , 7 ) , MnTMPyP ( 4 ) , phenylephrine ( 2 ) , PP2 ( 7 ) , Ro31-8220 ( 7 ) , RWPs ( 4 ) , tamoxifen ( 3 ) , TMB8 ( 5 ) , wortmannin ( 4 , 6 , 7 )

Downstream Regulation
Effects of modification on eNOS:
enzymatic activity, induced ( 5 )

Disease / Diagnostics Relevance
Relevant diseases:
stroke ( 2 )

References 

1

Loot AE, Schreiber JG, Fisslthaler B, Fleming I (2009) Angiotensin II impairs endothelial function via tyrosine phosphorylation of the endothelial nitric oxide synthase. J Exp Med 206, 2889-96
19934023   Curated Info

2

Atochin DN, et al. (2007) The phosphorylation state of eNOS modulates vascular reactivity and outcome of cerebral ischemia in vivo. J Clin Invest 117, 1961-7
17557122   Curated Info

3

Leung HS, et al. (2006) Tamoxifen dilates porcine coronary arteries: roles for nitric oxide and ouabain-sensitive mechanisms. Br J Pharmacol 149, 703-11
17016497   Curated Info

4

Ndiaye M, et al. (2005) Red wine polyphenol-induced, endothelium-dependent NO-mediated relaxation is due to the redox-sensitive PI3-kinase/Akt-dependent phosphorylation of endothelial NO-synthase in the isolated porcine coronary artery. FASEB J 19, 455-7
15623569   Curated Info

5

Anter E, et al. (2004) Activation of endothelial nitric-oxide synthase by the p38 MAPK in response to black tea polyphenols. J Biol Chem 279, 46637-43
15333638   Curated Info

6

Chen JX, et al. (2004) HSP90 and Akt modulate Ang-1-induced angiogenesis via NO in coronary artery endothelium. J Appl Physiol 96, 612-20
14555685   Curated Info

7

Thomas SR, Chen K, Keaney JF (2002) Hydrogen peroxide activates endothelial nitric-oxide synthase through coordinated phosphorylation and dephosphorylation via a phosphoinositide 3-kinase-dependent signaling pathway. J Biol Chem 277, 6017-24
11744698   Curated Info