Thr497
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Home > Phosphorylation Site Page: > Thr497  -  eNOS (pig)

Site Information
TGIARKKtFKEVANA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447885

In vivo Characterization
Methods used to characterize site in vivo:
phospho-antibody ( 1 , 2 , 3 ) , western blotting ( 1 , 2 , 3 )
Relevant cell line - cell type - tissue:
endothelial-aorta ( 2 ) , PAE (endothelial) ( 1 , 3 )

Upstream Regulation
Treatments:
AG1478 ( 3 ) , BAPTA-AM ( 3 ) , black_tea_polyphenols ( 2 ) , H2O2 ( 3 ) , LY294002 ( 3 ) , PP2 ( 3 ) , Ro31-8220 ( 3 ) , RWPs ( 1 ) , TMB8 ( 2 ) , wortmannin ( 3 )

Downstream Regulation
Effects of modification on eNOS:
enzymatic activity, inhibited ( 2 )

References 

1

Ndiaye M, et al. (2005) Red wine polyphenol-induced, endothelium-dependent NO-mediated relaxation is due to the redox-sensitive PI3-kinase/Akt-dependent phosphorylation of endothelial NO-synthase in the isolated porcine coronary artery. FASEB J 19, 455-7
15623569   Curated Info

2

Anter E, et al. (2004) Activation of endothelial nitric-oxide synthase by the p38 MAPK in response to black tea polyphenols. J Biol Chem 279, 46637-43
15333638   Curated Info

3

Thomas SR, Chen K, Keaney JF (2002) Hydrogen peroxide activates endothelial nitric-oxide synthase through coordinated phosphorylation and dephosphorylation via a phosphoinositide 3-kinase-dependent signaling pathway. J Biol Chem 277, 6017-24
11744698   Curated Info