Thr57
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Home > Phosphorylation Site Page: > Thr57  -  p21Cip1 (human)

Site Information
NFDFVtEtPLEGDFA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 451222

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 7 ) , mutation of modification site ( 2 , 3 , 4 , 5 , 6 , 7 , 8 ) , phosphopeptide mapping ( 7 ) , western blotting ( 6 )
Disease tissue studied:
breast cancer ( 3 ) , colorectal cancer ( 2 , 3 , 6 , 7 ) , colorectal carcinoma ( 2 , 3 , 6 , 7 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 5 , 6 ) , 3T3 (fibroblast) ( 5 ) , CHO (fibroblast) ( 4 ) , HaCaT (keratinocyte) ( 3 ) , HCT116 (intestinal) ( 2 , 6 ) , HD3 ( 7 ) , HeLa (cervical) ( 6 ) , HUVEC (endothelial) ( 8 )

Upstream Regulation
Regulatory protein:
MST1 (mouse) ( 5 ) , MST2 (mouse) ( 5 )
Putative in vivo kinases:
ERK2 (human) ( 6 ) , GSK3A (human) ( 3 )
Kinases, in vitro:
ERK2 (human) ( 1 , 6 ) , GSK3B (human) ( 8 ) , JNK1 (human) ( 1 , 5 , 7 ) , JNK2 (human) ( 1 ) , P38A (human) ( 7 )
Treatments:
botulinum_C3_toxin ( 5 ) , EGF ( 6 ) , U0126 ( 6 )

Downstream Regulation
Effects of modification on p21Cip1:
intracellular localization ( 6 ) , molecular association, regulation ( 4 ) , phosphorylation ( 4 ) , protein degradation ( 3 , 6 , 8 ) , protein stabilization ( 5 ) , ubiquitination ( 6 )
Effects of modification on biological processes:
apoptosis, induced ( 3 ) , cell cycle regulation ( 6 )
Inhibit interaction with:
CDK4 (human) ( 4 )

References 

1

Colleoni B, et al. (2017) JNKs function as CDK4-activating kinases by phosphorylating CDK4 and p21. Oncogene 36, 4349-4361
28368408   Curated Info

2

Rizzardi LF, et al. (2015) CDK1-dependent Inhibition of the E3 Ubiquitin Ligase CRL4CDT2 Ensures Robust Transition from S Phase to Mitosis. J Biol Chem 290, 556-67
25411249   Curated Info

3

Foertsch F, et al. (2013) S100A11 is involved in the regulation of the stability of cell cycle regulator p21(CIP) (1/) (WAF) (1) in human keratinocyte HaCaT cells. FEBS J 280, 3840-53
23745637   Curated Info

4

Bisteau X, et al. (2013) CDK4 T172 Phosphorylation Is Central in a CDK7-Dependent Bidirectional CDK4/CDK2 Interplay Mediated by p21 Phosphorylation at the Restriction Point. PLoS Genet 9, e1003546
23737759   Curated Info

5

Densham RM, et al. (2009) MST kinases monitor actin cytoskeletal integrity and signal via c-Jun N-terminal kinase stress-activated kinase to regulate p21Waf1/Cip1 stability. Mol Cell Biol 29, 6380-90
19822666   Curated Info

6

Hwang CY, Lee C, Kwon KS (2009) Extracellular signal-regulated kinase 2-dependent phosphorylation induces cytoplasmic localization and degradation of p21Cip1. Mol Cell Biol 29, 3379-89
19364816   Curated Info

7

Kim GY, et al. (2002) The stress-activated protein kinases p38 alpha and JNK1 stabilize p21(Cip1) by phosphorylation. J Biol Chem 277, 29792-802
12058028   Curated Info

8

Rössig L, et al. (2002) Glycogen synthase kinase-3 couples AKT-dependent signaling to the regulation of p21Cip1 degradation. J Biol Chem 277, 9684-9
11779850   Curated Info

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