Lys545
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Home > Ubiquitylation Site Page: > Lys545  -  Ku80 (human)

Site Information
PLIEAkkkDQVTAQE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 12344141

In vivo Characterization
Methods used to characterize site in vivo:
immunoassay ( 2 ) , immunoprecipitation ( 2 ) , mass spectrometry ( 1 , 3 ) , mutation of modification site ( 2 ) , western blotting ( 2 )
Disease tissue studied:
bone cancer ( 2 ) , lung cancer ( 2 ) , non-small cell lung cancer ( 2 )
Relevant cell line - cell type - tissue:

Downstream Regulation
Effects of modification on Ku80:
molecular association, regulation ( 2 )
Effects of modification on biological processes:
DNA repair, induced ( 2 )
Inhibit interaction with:
DNA ( 2 )

References 

1

Akimov V, et al. (2018) UbiSite approach for comprehensive mapping of lysine and N-terminal ubiquitination sites. Nat Struct Mol Biol 25, 631-640
29967540   Curated Info

2

Ishida N, et al. (2017) Ubiquitylation of Ku80 by RNF126 Promotes Completion of Nonhomologous End Joining-Mediated DNA Repair. Mol Cell Biol 37
27895153   Curated Info

3

Guo A (2009) CST Curation Set: 8645; Year: 2009; Biosample/Treatment: cell line, U266/Velcade; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG) Antibodies Used to Purify Peptides prior to LCMS: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info