Thr69
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Home > Phosphorylation Site Page: > Thr69  -  4E-BP1 (rat)

Site Information
RNsPVAKtPPKDLPT   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447528

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 2 , 10 ) , [32P] bio-synthetic labeling ( 9 , 10 , 12 ) , electrophoretic mobility shift ( 9 ) , mass spectrometry ( 1 ) , mutation of modification site ( 9 ) , phospho-antibody ( 2 , 3 , 4 , 5 , 6 , 7 , 8 ) , phosphoamino acid analysis ( 10 ) , phosphopeptide mapping ( 9 , 10 , 12 ) , western blotting ( 2 , 3 , 5 , 6 , 7 )
Relevant cell line - cell type - tissue:
'brain, cerebral cortex' ( 2 ) , 'brain, hippocampus' ( 2 ) , 'muscle, skeletal' ( 4 , 5 ) , 293 (epithelial) ( 6 , 9 ) , adipocyte-adipose tissue ( 10 , 12 ) , adipose tissue ( 1 ) , heart ( 1 ) , IEC-18 (epithelial) ( 3 ) , intestine ( 1 ) , liver ( 1 , 8 ) , lung ( 1 ) , muscle ( 1 ) , pancreas ( 1 ) , spleen ( 1 ) , thymus ( 1 ) , vascular smooth muscle cell ('muscle, smooth') ( 7 )

Upstream Regulation
Kinases, in vitro:
ERK2 (human) ( 11 , 12 ) , mTOR (rat) ( 11 )
Treatments:
amino_acids ( 5 , 9 ) , angiotensin ( 7 ) , ethanol ( 5 ) , exercise ( 4 ) , insulin ( 6 , 9 , 10 , 12 ) , ischemia ( 2 ) , ischemia/reperfusion ( 2 ) , LY294002 ( 7 ) , partial_hepatectomy ( 8 ) , phorbol_ester ( 3 ) , rapamycin ( 3 , 4 , 6 , 8 , 9 , 10 , 12 ) , U0126 ( 7 )

Downstream Regulation
Effects of modification on 4E-BP1:
molecular association, regulation ( 2 , 9 , 10 ) , phosphorylation ( 9 )
Effects of modification on biological processes:
translation, altered ( 2 , 9 )
Induce interaction with:
EIF4E (rat) ( 2 )
Inhibit interaction with:
EIF4E (human) ( 9 ) , EIF4E (rat) ( 10 )

References 

1

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

2

Ayuso MI, et al. (2010) New hierarchical phosphorylation pathway of the translational repressor eIF4E-binding protein 1 (4E-BP1) in ischemia-reperfusion stress. J Biol Chem 285, 34355-63
20736160   Curated Info

3

Guan L, et al. (2007) Protein kinase C-mediated down-regulation of cyclin D1 involves activation of the translational repressor 4E-BP1 via a phosphoinositide 3-kinase/Akt-independent, protein phosphatase 2A-dependent mechanism in intestinal epithelial cells. J Biol Chem 282, 14213-25
17360714   Curated Info

4

Kubica N, et al. (2005) Resistance exercise increases muscle protein synthesis and translation of eukaryotic initiation factor 2Bepsilon mRNA in a mammalian target of rapamycin-dependent manner. J Biol Chem 280, 7570-80
15591312   Curated Info

5

Lang CH, et al. (2003) Alcohol impairs leucine-mediated phosphorylation of 4E-BP1, S6K1, eIF4G, and mTOR in skeletal muscle. Am J Physiol Endocrinol Metab 285, E1205-15
12944322   Curated Info

6

Ferguson G, Mothe-Satney I, Lawrence JC (2003) Ser-64 and Ser-111 in PHAS-I are dispensable for insulin-stimulated dissociation from eIF4E. J Biol Chem 278, 47459-65
14507920   Curated Info

7

Rocic P, Seshiah P, Griendling KK (2003) Reactive oxygen species sensitivity of angiotensin II-dependent translation initiation in vascular smooth muscle cells. J Biol Chem 278, 36973-9
12860993   Curated Info

8

Jiang YP, Ballou LM, Lin RZ (2001) Rapamycin-insensitive regulation of 4e-BP1 in regenerating rat liver. J Biol Chem 276, 10943-51
11278364   Curated Info

9

Mothe-Satney I, et al. (2000) Multiple mechanisms control phosphorylation of PHAS-I in five (S/T)P sites that govern translational repression. Mol Cell Biol 20, 3558-67
10779345   Curated Info

10

Heesom KJ, Avison MB, Diggle TA, Denton RM (1998) Insulin-stimulated kinase from rat fat cells that phosphorylates initiation factor 4E-binding protein 1 on the rapamycin-insensitive site (serine-111). Biochem J 336 ( Pt 1), 39-48
9806882   Curated Info

11

Brunn GJ, Fadden P, Haystead TA, Lawrence JC (1997) The mammalian target of rapamycin phosphorylates sites having a (Ser/Thr)-Pro motif and is activated by antibodies to a region near its COOH terminus. J Biol Chem 272, 32547-50
9405468   Curated Info

12

Fadden P, Haystead TA, Lawrence JC (1997) Identification of phosphorylation sites in the translational regulator, PHAS-I, that are controlled by insulin and rapamycin in rat adipocytes. J Biol Chem 272, 10240-7
9092573   Curated Info