Ser555
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Home > Phosphorylation Site Page: > Ser555  -  NHE3 (human)

Site Information
AEGERRGsLAFIRSP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 450899

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 5 , 6 , 7 , 8 ) , mutation of modification site ( 1 ) , phospho-antibody ( 1 , 2 , 4 ) , western blotting ( 1 , 2 , 4 )
Disease tissue studied:
colorectal cancer ( 1 ) , colorectal carcinoma ( 1 ) , lung cancer ( 5 ) , non-small cell lung cancer ( 5 ) , melanoma skin cancer ( 6 , 7 , 8 )
Relevant cell line - cell type - tissue:
C2BBe1 (intestinal) ( 1 ) , epithelial-'kidney, tubule, proximal' ( 4 ) , lung ( 5 ) , NT16 (renal tubule cell) ( 4 ) , OKP (epithelial) ( 2 ) , skin ( 6 , 7 , 8 )

Upstream Regulation
Regulatory protein:
PIK3CA (human) ( 4 )
Putative in vivo kinases:
AMPKA1 (human) ( 1 ) , AMPKA2 (human) ( 1 )
Treatments:
acadesine ( 1 ) , angiotensin_2 ( 2 ) , BAPTA-AM ( 4 ) , chelerythrine ( 4 ) , colforsin ( 2 ) , gastrin ( 4 ) , Go_6976 ( 4 ) , H-89 ( 1 ) , losartan ( 2 ) , NKH_477 ( 1 ) , PTX ( 2 ) , siRNA ( 1 ) , U73122 ( 4 ) , wortmannin ( 4 )

Downstream Regulation
Effects of modification on NHE3:
activity, inhibited ( 1 , 4 ) , receptor internalization, induced ( 4 ) , ubiquitination ( 1 )
Effects of modification on biological processes:
endocytosis, induced ( 4 ) , signaling pathway regulation ( 4 )

References 

1

Han Y, Bagchi P, Yun CC (2024) Regulation of the intestinal Na/H exchanger NHE3 by AMP-activated kinase is dependent on phosphorylation of NHE3 at S555 and S563. Am J Physiol Cell Physiol 326, C50-C59
38047302   Curated Info

2

Crajoinas RO, et al. (2016) Angiotensin II counteracts the effects of cAMP/PKA on NHE3 activity and phosphorylation in proximal tubule cells. Am J Physiol Cell Physiol 311, C768-C776
27510906   Curated Info

3

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

4

Liu T, Jose PA (2013) Gastrin Induces Sodium-Hydrogen Exchanger 3 Phosphorylation and mTOR Activation via a Phosphoinositide 3-Kinase-/Protein Kinase C-Dependent but AKT-Independent Pathway in Renal Proximal Tubule Cells Derived From a Normotensive Male Human. Endocrinology 154, 865-75
23275470   Curated Info

5

Rikova K (2010) CST Curation Set: 9010; Year: 2010; Biosample/Treatment: tissue, lung/untreated; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

6

Tucker M (2009) CST Curation Set: 8377; Year: 2009; Biosample/Treatment: tissue, skin/untreated; Disease: melanoma skin cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

7

Tucker M (2009) CST Curation Set: 8385; Year: 2009; Biosample/Treatment: tissue, skin/untreated; Disease: melanoma skin cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

8

Tucker M (2009) CST Curation Set: 6432; Year: 2009; Biosample/Treatment: tissue, skin/untreated; Disease: melanoma skin cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

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