Ser563
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Home > Phosphorylation Site Page: > Ser563  -  NHE3 (human)

Site Information
LAFIRSPstDNVVNV   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 12265540

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 ) , mass spectrometry ( 1 , 2 , 3 , 4 ) , mutation of modification site ( 1 )
Disease tissue studied:
colorectal cancer ( 1 ) , colorectal carcinoma ( 1 ) , lung cancer ( 2 ) , non-small cell lung cancer ( 2 ) , melanoma skin cancer ( 3 , 4 )
Relevant cell line - cell type - tissue:
C2BBe1 (intestinal) ( 1 ) , lung ( 2 ) , skin ( 3 , 4 )

Upstream Regulation
Putative in vivo kinases:
AMPKA1 (human) ( 1 )
Treatments:
acadesine ( 1 )

Downstream Regulation
Effects of modification on NHE3:
activity, inhibited ( 1 ) , ubiquitination ( 1 )

References 

1

Han Y, Bagchi P, Yun CC (2024) Regulation of the intestinal Na/H exchanger NHE3 by AMP-activated kinase is dependent on phosphorylation of NHE3 at S555 and S563. Am J Physiol Cell Physiol 326, C50-C59
38047302   Curated Info

2

Rikova K (2010) CST Curation Set: 9010; Year: 2010; Biosample/Treatment: tissue, lung/untreated; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

3

Tucker M (2009) CST Curation Set: 8377; Year: 2009; Biosample/Treatment: tissue, skin/untreated; Disease: melanoma skin cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

4

Tucker M (2009) CST Curation Set: 8385; Year: 2009; Biosample/Treatment: tissue, skin/untreated; Disease: melanoma skin cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

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