Ser118
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Home > Phosphorylation Site Page: > Ser118  -  TEBP (human)

Site Information
DDsDEDMsNFDRFsE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 451130

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 14 ) , immunoprecipitation ( 14 ) , mass spectrometry ( 1 , 2 , 3 , 4 , 5 , 7 , 8 , 9 , 10 , 11 , 12 , 13 ) , mutation of modification site ( 14 ) , western blotting ( 14 )
Disease tissue studied:
HER2 positive breast cancer ( 1 ) , luminal A breast cancer ( 1 ) , luminal B breast cancer ( 1 ) , breast cancer, triple negative ( 1 ) , cervical cancer ( 7 ) , cervical adenocarcinoma ( 7 ) , lung cancer ( 9 ) , non-small cell lung cancer ( 9 ) , melanoma skin cancer ( 2 )
Relevant cell line - cell type - tissue:
293 (epithelial) [ADRB1 (human), no information, overexpresses human beta1-adrenergic (ß1AR- HEK293)] ( 11 ) , 3Y1 (fibroblast) ( 14 ) , breast ( 1 ) , COS (fibroblast) ( 12 ) , Flp-In T-Rex-293 (epithelial) [PRKD1 (human), genetic knockin] ( 5 ) , Flp-In T-Rex-293 (epithelial) ( 5 ) , HeLa (cervical) ( 10 , 13 ) , HeLa S3 (cervical) ( 7 ) , Jurkat (T lymphocyte) ( 4 , 8 ) , liver ( 3 ) , NCI-H358 (pulmonary) ( 9 ) , WM239A (melanocyte) ( 2 )

Upstream Regulation
Putative in vivo kinases:
CK2A1 (human) ( 14 )
Kinases, in vitro:
CK2A1 (human) ( 14 )
Treatments:
A23187 ( 14 ) , arachidonic_acid ( 14 ) , bradykinin ( 14 ) , nocodazole ( 7 )

Downstream Regulation
Effects of modification on TEBP:
enzymatic activity, induced ( 14 ) , molecular association, regulation ( 14 )
Induce interaction with:
CK2A1 (human) ( 14 ) , HSP90A (human) ( 14 )

References 

1

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

2

Stuart SA, et al. (2015) A Phosphoproteomic Comparison of B-RAFV600E and MKK1/2 Inhibitors in Melanoma Cells. Mol Cell Proteomics 14, 1599-615
25850435   Curated Info

3

Bian Y, et al. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics 96, 253-62
24275569   Curated Info

4

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

5

Franz-Wachtel M, et al. (2012) Global detection of protein kinase D-dependent phosphorylation events in nocodazole-treated human cells. Mol Cell Proteomics 11, 160-70
22496350   Curated Info

6

Beli P, et al. (2012) Proteomic Investigations Reveal a Role for RNA Processing Factor THRAP3 in the DNA Damage Response. Mol Cell 46, 212-25
22424773   Curated Info

7

Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3
20068231   Curated Info

8

Possemato A (2009) CST Curation Set: 7867; Year: 2009; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

9

Rikova K (2009) CST Curation Set: 6083; Year: 2009; Biosample/Treatment: cell line, NCI-H358/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

10

Zhou J (2008) CST Curation Set: 4778; Year: 2008; Biosample/Treatment: cell line, HeLa/UV; Disease: cervical adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]Q Antibodies Used to Purify Peptides prior to LCMS: Phospho-ATM/ATR Substrate Motif (pS/pTQG) Antibody Cat#: 5074, PTMScan(R) Phospho-ATM/ATR Substrate Motif (pS/pTQG) Immunoaffinity Beads Cat#: 1993
Curated Info

11

Ruse CI, et al. (2008) Motif-specific sampling of phosphoproteomes. J Proteome Res 7, 2140-50
18452278   Curated Info

12

Wang Y, et al. (2007) Profiling signaling polarity in chemotactic cells. Proc Natl Acad Sci U S A 104, 8328-33
17494752   Curated Info

13

Olsen JV, et al. (2006) Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Cell 127, 635-48
17081983   Curated Info

14

Kobayashi T, et al. (2004) Regulation of cytosolic prostaglandin E synthase by phosphorylation. Biochem J 381, 59-69
15040786   Curated Info

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