Ser47

Site Information
PRRVRRFsGKAEPRP SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 1211090

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 2 ) , phospho-antibody ( 1 ) , western blotting ( 1 )
Relevant cell line - cell type - tissue:	3T3-L1 (fibroblast) ( 2 ) , COS7 (fibroblast) ( 1 ) , HEK293T (epithelial) ( 1 )

Upstream Regulation
Treatments:	angiotensin_2 ( 1 ) , bisindolylmaleimide ( 1 ) , colforsin ( 1 ) , H-89 ( 1 ) , phorbol_ester ( 1 )

References
1	Castañeda-Bueno M, et al. (2017) Phosphorylation by PKC and PKA regulate the kinase activity and downstream signaling of WNK4. Proc Natl Acad Sci U S A 114, E879-E886 28096417 Curated Info
2	Possemato A (2008) CST Curation Set: 4747; Year: 2008; Biosample/Treatment: cell line, 3T3-L1/Insulin; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS/T) Immunoaffinity Beads Cat#: 1978 Curated Info

References

Castañeda-Bueno M, et al. (2017) Phosphorylation by PKC and PKA regulate the kinase activity and downstream signaling of WNK4. Proc Natl Acad Sci U S A 114, E879-E886
28096417 Curated Info

Possemato A (2008) CST Curation Set: 4747; Year: 2008; Biosample/Treatment: cell line, 3T3-L1/Insulin; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info