Ser216
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Home > Phosphorylation Site Page: > Ser216  -  ER-alpha (mouse)

Site Information
CKAFFKRsIQGHNDY   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 12127402

In vivo Characterization
Methods used to characterize site in vivo:
immunoassay ( 1 , 3 ) , immunoprecipitation ( 2 ) , mutation of modification site ( 1 , 2 , 3 , 4 ) , phospho-antibody ( 1 , 2 , 3 , 4 ) , western blotting ( 1 , 3 , 4 )
Disease tissue studied:
liver cancer ( 2 ) , hepatocellular carcinoma ( 2 )
Relevant cell line - cell type - tissue:
'brain, substantia nigra' ( 1 ) , astrocyte ( 1 ) , glial-brain ( 1 ) , Huh7 (hepatic) ( 2 ) , liver ( 2 ) , microglia ( 1 ) , neutrophil ( 4 ) , neutrophil-blood ( 3 ) , uterus ( 3 , 4 ) , WBC-blood ( 4 )

Upstream Regulation
Kinases, in vitro:
PKCA (mouse) ( 3 , 4 )
Treatments:
17-beta-estradiol ( 4 ) , phenobarbital ( 2 )

Downstream Regulation
Effects of modification on ER-alpha:
molecular association, regulation ( 2 )
Effects of modification on biological processes:
apoptosis, inhibited ( 1 ) , cell motility, induced ( 3 , 4 ) , transcription, induced ( 2 ) , transcription, inhibited ( 1 )
Induce interaction with:
CaR (mouse) ( 2 ) , DNA ( 2 )

References 

1

Shindo S, et al. (2020) Estrogen receptor α phosphorylated at Ser216 confers inflammatory function to mouse microglia. Cell Commun Signal 18, 117
32727504   Curated Info

2

Yi M, et al. (2020) Nuclear receptor CAR-ERα signaling regulates the estrogen sulfotransferase gene in the liver. Sci Rep 10, 5001
32193417   Curated Info

3

Shindo S, Moore R, Negishi M (2016) Detection and Functional Analysis of Estrogen Receptor α Phosphorylated at Serine 216 in Mouse Neutrophils. Methods Mol Biol 1366, 413-24
26585153   Curated Info

4

Shindo S, Moore R, Flake G, Negishi M (2013) Serine 216 Phosphorylation of Estrogen Receptor α in Neutrophils: Migration and Infiltration into the Mouse Uterus. PLoS One 8, e84462
24386386   Curated Info

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