Ser187
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.6.0.2
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser187  -  SNAP-25 (mouse)

Site Information
RIMEkADsNkTRIDE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448668

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 4 ) , immunoprecipitation ( 5 ) , mutation of modification site ( 1 , 2 , 3 , 5 ) , phospho-antibody ( 3 , 4 ) , western blotting ( 4 , 5 )
Relevant cell line - cell type - tissue:
'neuron, cortical'-brain ( 3 ) , 'neuron, hippocampal'-brain ( 3 , 4 ) , brain [SNAP-25 (mouse), genetic knockin] ( 2 ) , chromaffin-adrenal gland ( 5 )

Upstream Regulation
Putative in vivo kinases:
PKCA (mouse) ( 3 , 4 , 5 )
Treatments:
bisindolylmaleimide ( 3 , 5 ) , Ca(2+) ( 5 ) , depolarization ( 3 ) , Go_6976 ( 5 ) , kainic_acid ( 3 ) , KCl ( 5 ) , morphine ( 4 ) , naloxone ( 4 ) , phorbol_ester ( 3 , 5 )

Downstream Regulation
Effects of modification on SNAP-25:
activity, inhibited ( 3 )
Effects of modification on biological processes:
exocytosis, altered ( 5 ) , neural plasticity ( 1 )

References 

1

Katayama N, et al. (2017) SNAP-25 phosphorylation at Ser187 regulates synaptic facilitation and short-term plasticity in an age-dependent manner. Sci Rep 7, 7996
28801590   Curated Info

2

Kataoka M, et al. (2011) A single amino acid mutation in SNAP-25 induces anxiety-related behavior in mouse. PLoS One 6, e25158
21949876   Curated Info

3

Pozzi D, et al. (2008) Activity-dependent phosphorylation of Ser187 is required for SNAP-25-negative modulation of neuronal voltage-gated calcium channels. Proc Natl Acad Sci U S A 105, 323-8
18162553   Curated Info

4

Xu NJ, et al. (2004) Inhibition of SNAP-25 phosphorylation at Ser187 is involved in chronic morphine-induced down-regulation of SNARE complex formation. J Biol Chem 279, 40601-8
15277518   Curated Info

5

Nagy G, et al. (2002) Protein kinase C-dependent phosphorylation of synaptosome-associated protein of 25 kDa at Ser187 potentiates vesicle recruitment. J Neurosci 22, 9278-86
12417653   Curated Info