Thr138
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Home > Phosphorylation Site Page: > Thr138  -  SNAP-25 (mouse)

Site Information
GGFIRRVtNDARENE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 449875
Associated spectra:  2 CST

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 ) , mass spectrometry ( 1 , 2 , 3 , 4 , 5 , 6 ) , mutation of modification site ( 7 ) , phospho-antibody ( 1 , 7 ) , western blotting ( 1 )
Disease tissue studied:
neuroblastoma ( 1 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
PKACA (mouse) ( 7 ) , ROCK2 (rat) ( 1 )
Kinases, in vitro:
ROCK2 (human) ( 1 )
Putative upstream phosphatases:
MYPT1 (rat) ( 1 )
Phosphatases, in vitro:
PPP1CA (human) ( 1 )
Treatments:
cAMP_analog ( 7 ) , colforsin ( 7 ) , IBMX ( 7 ) , KT5720 ( 7 ) , PDGF ( 3 )

Downstream Regulation
Effects of modification on SNAP-25:
molecular association, regulation ( 1 )
Effects of modification on biological processes:
exocytosis, inhibited ( 1 )
Inhibit interaction with:
STX1A (mouse) ( 1 )

References 

1

Horváth D, et al. (2017) Myosin phosphatase and RhoA-activated kinase modulate neurotransmitter release by regulating SNAP-25 of SNARE complex. PLoS One 12, e0177046
28486561   Curated Info

2

Goswami T, et al. (2012) Comparative phosphoproteomic analysis of neonatal and adult murine brain. Proteomics 12, 2185-9
22807455   Curated Info

3

Biarc J, Chalkley RJ, Burlingame AL, Bradshaw RA (2012) The induction of serine/threonine protein phosphorylations by a PDGFR/TrkA chimera in stably transfected PC12 cells. Mol Cell Proteomics 11, 15-30
22027198   Curated Info

4

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

5

Guo A (2007) CST Curation Set: 3229; Year: 2007; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

6

Guo A (2007) CST Curation Set: 3031; Year: 2007; Biosample/Treatment: tissue, brain/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PKA Substrate Antibody Cat#: 9621
Curated Info

7

Nagy G, et al. (2004) Regulation of releasable vesicle pool sizes by protein kinase A-dependent phosphorylation of SNAP-25. Neuron 41, 417-29
14766180   Curated Info