Ser39
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Home > Phosphorylation Site Page: > Ser39  -  fascin (human)

Site Information
KVNASAssLkkkQIW   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 450888

In vivo Characterization
Methods used to characterize site in vivo:
immunoassay ( 2 ) , mass spectrometry ( 1 , 4 , 7 , 8 , 9 , 11 ) , mutation of modification site ( 2 , 3 , 5 , 6 , 13 ) , phospho-antibody ( 2 , 12 ) , western blotting ( 3 , 5 , 6 )
Disease tissue studied:
HER2 positive breast cancer ( 7 ) , luminal A breast cancer ( 7 ) , luminal B breast cancer ( 7 ) , breast cancer, triple negative ( 7 ) , esophageal cancer ( 5 , 12 ) , esophageal carcinoma ( 5 , 12 ) , lung cancer ( 9 ) , non-small cell lung adenocarcinoma ( 9 ) , neuroblastoma ( 1 , 3 ) , cancer, squamous cell carcinoma ( 5 , 12 )
Relevant cell line - cell type - tissue:
A375MM (melanocyte) ( 13 ) , A549 (pulmonary) [CD38 (human), transfection, Lentiviral particles containing CD38 vector were transfected] ( 4 ) , A549 (pulmonary) ( 4 ) , breast ( 7 ) , CLB-Bar ( 1 ) , EC109 ( 12 ) , EC171 ( 12 ) , EC8712 ( 12 ) , EC9706 ( 12 ) , esophagus ( 12 ) , HeLa (cervical) ( 2 , 6 , 8 ) , HUES-9 ('stem, embryonic') ( 11 ) , Kyse150 (esophageal) ( 5 , 12 ) , Kyse180 (esophageal) ( 5 , 12 ) , Kyse510 (esophageal) ( 12 ) , lung ( 9 ) , NG108-15 (neuron) ( 3 ) , SHEEC ( 12 )

Upstream Regulation
Putative in vivo kinases:
PKCA (human) ( 2 , 3 )
Kinases, in vitro:
PKCA (human) ( 14 )
Treatments:
bisindolylmaleimide ( 2 ) , phorbol_ester ( 3 )

Downstream Regulation
Effects of modification on fascin:
intracellular localization ( 2 , 3 , 6 ) , molecular association, regulation ( 2 , 14 ) , protein conformation ( 2 )
Effects of modification on biological processes:
cell growth, inhibited ( 5 ) , cell motility, inhibited ( 5 ) , cytoskeletal reorganization ( 2 , 3 , 5 , 13 )
Induce interaction with:
ACTA1 (human) ( 14 )
Inhibit interaction with:
ACTA1 (human) ( 2 ) , FMNL2 (human) ( 2 )

Disease / Diagnostics Relevance
Relevant diseases:
cancer, squamous cell carcinoma ( 12 )

References 

1

Borenäs M, et al. (2024) ALK signaling primes the DNA damage response sensitizing ALK-driven neuroblastoma to therapeutic ATR inhibition. Proc Natl Acad Sci U S A 121, e2315242121
38154064   Curated Info

2

Pfisterer K, et al. (2020) FMNL2 regulates dynamics of fascin in filopodia. J Cell Biol 219
32294157   Curated Info

3

Tanaka M, et al. (2019) Fascin in lamellipodia contributes to cell elasticity by controlling the orientation of filamentous actin. Genes Cells
30664308   Curated Info

4

Wang W, et al. (2018) Decreased NAD Activates STAT3 and Integrin Pathways to Drive Epithelial-Mesenchymal Transition. Mol Cell Proteomics
29980616   Curated Info

5

Zeng FM, et al. (2017) Fascin phosphorylation sites combine to regulate esophageal squamous cancer cell behavior. Amino Acids 49, 943-955
28251354   Curated Info

6

Jayo A, et al. (2016) Fascin Regulates Nuclear Movement and Deformation in Migrating Cells. Dev Cell 38, 371-83
27554857   Curated Info

7

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

8

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

9

Schweppe DK, Rigas JR, Gerber SA (2013) Quantitative phosphoproteomic profiling of human non-small cell lung cancer tumors. J Proteomics 91, 286-96
23911959   Curated Info

10

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

11

Rigbolt KT, et al. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal 4, rs3
21406692   Curated Info

12

Zhao Q, et al. (2010) Phosphorylation of fascin decreases the risk of poor survival in patients with esophageal squamous cell carcinoma. J Histochem Cytochem 58, 979-88
20713986   Curated Info

13

Li A, et al. (2010) The actin-bundling protein fascin stabilizes actin in invadopodia and potentiates protrusive invasion. Curr Biol 20, 339-45
20137952   Curated Info

14

Ono S, et al. (1997) Identification of an actin binding region and a protein kinase C phosphorylation site on human fascin. J Biol Chem 272, 2527-33
8999969   Curated Info

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