Ser246

Site Information
PGETPPLsPIDMESQ SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 450451

In vivo Characterization
Methods used to characterize site in vivo:	[32P] bio-synthetic labeling ( 2 ) , immunoprecipitation ( 2 ) , mass spectrometry ( 1 ) , mutation of modification site ( 2 )
Relevant cell line - cell type - tissue:	208F ( 2 ) , IEC-6 (epithelial) ( 1 )

Upstream Regulation
Kinases, in vitro:	CDK1 (human) ( 2 ) , ERK1 (rat) ( 2 ) , ERK2 (human) ( 3 )
Treatments:	serum ( 1 , 2 )

References
1	Courcelles M, et al. (2013) Phosphoproteome dynamics reveal novel ERK1/2 MAP kinase substrates with broad spectrum of functions. Mol Syst Biol 9, 669 23712012 Curated Info
2	Baker SJ, et al. (1992) Jun is phosphorylated by several protein kinases at the same sites that are modified in serum-stimulated fibroblasts. Mol Cell Biol 12, 4694-705 1328860 Curated Info
3	Alvarez E, et al. (1991) Pro-Leu-Ser/Thr-Pro is a consensus primary sequence for substrate protein phosphorylation. Characterization of the phosphorylation of c-myc and c-jun proteins by an epidermal growth factor receptor threonine 669 protein kinase. J Biol Chem 266, 15277-85 1651323 Curated Info