Ser556
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Home > Phosphorylation Site Page: > Ser556  -  FANCI (human)

Site Information
VLGSLSSsQCsQSLS   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 11952934

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 4 ) , mass spectrometry ( 4 ) , mass spectrometry (in vitro) ( 1 ) , mutation of modification site ( 3 ) , phospho-antibody ( 2 , 3 , 4 ) , western blotting ( 2 , 3 , 4 )
Disease tissue studied:
bone cancer ( 2 ) , ovarian cancer ( 2 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
ATR (human) ( 3 ) , FANCD2 (human) ( 3 )
Putative in vivo kinases:
ATM (human) ( 4 )
Kinases, in vitro:
ATR (human) ( 1 )
Treatments:
hydroxyurea ( 3 , 4 ) , ionizing_radiation ( 4 ) , mitomycin_C ( 3 ) , VE-821 ( 3 )

Downstream Regulation
Effects of modification on FANCI:
molecular association, regulation ( 1 , 3 ) , ubiquitination ( 1 )
Effects of modification on biological processes:
cell growth, inhibited ( 3 ) , DNA repair, induced ( 3 )
Induce interaction with:
DNA ( 1 ) , FANCD2 (human) ( 3 )
Inhibit interaction with:
MCM5 (human) ( 3 )

References 

1

Tan W, van Twest S, Murphy VJ, Deans AJ (2020) ATR-Mediated FANCI Phosphorylation Regulates Both Ubiquitination and Deubiquitination of FANCD2. Front Cell Dev Biol 8, 2
32117957   Curated Info

2

Cheung RS, et al. (2017) Ubiquitination-Linked Phosphorylation of the FANCI S/TQ Cluster Contributes to Activation of the Fanconi Anemia I/D2 Complex. Cell Rep 19, 2432-2440
28636932   Curated Info

3

Chen YH, et al. (2015) ATR-Mediated Phosphorylation of FANCI Regulates Dormant Origin Firing in Response to Replication Stress. Mol Cell 58, 323-38
25843623   Curated Info

4

Mu JJ, et al. (2007) A proteomic analysis of ataxia telangiectasia-mutated (ATM)/ATM-Rad3-related (ATR) substrates identifies the ubiquitin-proteasome system as a regulator for DNA damage checkpoints. J Biol Chem 282, 17330-4
17478428   Curated Info