Ser1176
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Home > Phosphorylation Site Page: > Ser1176  -  eNOS (rat)

Site Information
TSRIRtQsFsLQERQ   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447884

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 6 ) , phospho-antibody ( 1 , 2 , 3 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 20 , 21 , 22 , 23 , 24 ) , western blotting ( 1 , 2 , 3 , 8 , 9 , 11 , 12 , 14 , 15 , 17 , 18 , 20 , 21 , 22 )
Relevant cell line - cell type - tissue:
adipocyte-adipose tissue ( 13 ) , adipose tissue ( 6 ) , aorta ( 18 , 21 ) , BAEC (endothelial) ( 10 ) , endothelial-aorta ( 15 , 22 ) , endothelial-liver ( 3 ) , HAEC (endothelial) ( 21 ) , heart ( 6 , 9 , 24 ) , kidney ( 8 , 10 , 11 , 14 ) , liver ( 6 ) , lung ( 2 ) , microvessel endothelial-'brain, forebrain' ( 16 ) , penis ( 12 ) , pulmonary artery ( 17 ) , RAEC (endothelial) ( 1 ) , spleen ( 6 ) , stomach ( 6 ) , vascular ( 23 ) , vein ( 20 )

Upstream Regulation
Regulatory protein:
GIT1 (rat) ( 3 ) , GRK2 (rat) ( 3 )
Putative in vivo kinases:
Akt1 (rat) ( 3 , 16 ) , AMPKA1 (rat) ( 24 )
Kinases, in vitro:
AMPKA1 (rat) ( 24 ) , PKACA (human) ( 19 )
Treatments:
ACh ( 15 ) , antimiR-199a ( 1 ) , atorvastatin ( 22 ) , beta-amyloid_40 ( 15 ) , bradykinin ( 9 ) , caffeine ( 2 ) , cAMP_analog ( 13 ) , deoxygenation ( 1 ) , endothelin ( 3 , 8 ) , fluid_shear_stress ( 12 ) , Go_6976 ( 15 ) , H-89 ( 13 ) , high-salt diet ( 11 ) , hyperglycemia ( 23 ) , hyperoxia ( 2 ) , hypertension ( 17 , 20 ) , insulin ( 9 , 13 , 18 ) , ischemia ( 9 , 24 ) , ischemia/reperfusion ( 16 ) , L-NAME ( 1 ) , leptin ( 13 , 18 , 21 ) , LY294002 ( 9 ) , okadaic_acid ( 13 ) , PP2 ( 3 ) , PTX ( 3 ) , Safflor yellow B ( 1 ) , tautomycin ( 13 ) , U0126 ( 13 ) , wortmannin ( 8 )

Downstream Regulation
Effects of modification on eNOS:
enzymatic activity, induced ( 24 )

Disease / Diagnostics Relevance
Relevant diseases:
diabetes mellitus ( 12 )

References 

1

Wang C, et al. (2017) Safflor yellow B reduces hypoxia-mediated vasoconstriction by regulating endothelial micro ribonucleic acid/nitric oxide synthase signaling. Oncotarget 8, 93551-93566
29212172   Curated Info

2

Jing X, et al. (2017) Caffeine ameliorates hyperoxia-induced lung injury by protecting GCH1 function in neonatal rat pups. Pediatr Res 82, 483-489
28399119   Curated Info

3

Liu S, Premont RT, Rockey DC (2014) Endothelial nitric-oxide synthase (eNOS) is activated through G-protein-coupled receptor kinase-interacting protein 1 (GIT1) tyrosine phosphorylation and Src protein. J Biol Chem 289, 18163-74
24764294   Curated Info

4

Oelze M, et al. (2014) The sodium-glucose co-transporter 2 inhibitor empagliflozin improves diabetes-induced vascular dysfunction in the streptozotocin diabetes rat model by interfering with oxidative stress and glucotoxicity. PLoS One 9, e112394
25402275   Curated Info

5

Tajbakhsh N, Sokoya EM (2013) Sirtuin 1 is upregulated in young obese Zucker rat cerebral arteries. Eur J Pharmacol 721, 43-8
24113524   Curated Info

6

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

7

Angelone T, et al. (2011) Distinct signalling mechanisms are involved in the dissimilar myocardial and coronary effects elicited by quercetin and myricetin, two red wine flavonols. Nutr Metab Cardiovasc Dis 21, 362-71
20096547   Curated Info

8

Herrera M, Hong NJ, Ortiz PA, Garvin JL (2009) Endothelin-1 inhibits thick ascending limb transport via Akt-stimulated nitric oxide production. J Biol Chem 284, 1454-60
19033447   Curated Info

9

Kaiserova K, Tang XL, Srivastava S, Bhatnagar A (2008) Role of nitric oxide in regulating aldose reductase activation in the ischemic heart. J Biol Chem 283, 9101-12
18223294   Curated Info

10

Mount PF, et al. (2006) Phosphorylation of neuronal and endothelial nitric oxide synthase in the kidney with high and low salt diets. Nephron Physiol 102, p36-50
16244499   Curated Info

11

Herrera M, Silva G, Garvin JL (2006) A high-salt diet dissociates NO synthase-3 expression and NO production by the thick ascending limb. Hypertension 47, 95-101
16344378   Curated Info

12

Musicki B, Kramer MF, Becker RE, Burnett AL (2005) Inactivation of phosphorylated endothelial nitric oxide synthase (Ser-1177) by O-GlcNAc in diabetes-associated erectile dysfunction. Proc Natl Acad Sci U S A 102, 11870-5
16085713   Curated Info

13

Mehebik N, et al. (2005) Leptin-induced nitric oxide production in white adipocytes is mediated through PKA and MAP kinase activation. Am J Physiol Cell Physiol 289, C379-87
15772123   Curated Info

14

Lee DL, et al. (2005) Posttranslational regulation of NO synthase activity in the renal medulla of diabetic rats. Am J Physiol Renal Physiol 288, F82-90
15383397   Curated Info

15

Gentile MT, et al. (2004) Mechanisms of soluble beta-amyloid impairment of endothelial function. J Biol Chem 279, 48135-42
15319431   Curated Info

16

Osuka K, et al. (2004) Modification of endothelial NO synthase through protein phosphorylation after forebrain cerebral ischemia/reperfusion. Stroke 35, 2582-6
15375304   Curated Info

17

Sasser JM, et al. (2004) Reduced NOS3 phosphorylation mediates reduced NO/cGMP signaling in mesenteric arteries of deoxycorticosterone acetate-salt hypertensive rats. Hypertension 43, 1080-5
14993198   Curated Info

18

Vecchione C, et al. (2003) Cooperation between insulin and leptin in the modulation of vascular tone. Hypertension 42, 166-70
12835332   Curated Info

19

Michell BJ, et al. (2002) Identification of regulatory sites of phosphorylation of the bovine endothelial nitric-oxide synthase at serine 617 and serine 635. J Biol Chem 277, 42344-51
12171920   Curated Info

20

Iwakiri Y, et al. (2002) Phosphorylation of eNOS initiates excessive NO production in early phases of portal hypertension. Am J Physiol Heart Circ Physiol 282, H2084-90
12003815   Curated Info

21

Vecchione C, et al. (2002) Leptin effect on endothelial nitric oxide is mediated through Akt-endothelial nitric oxide synthase phosphorylation pathway. Diabetes 51, 168-73
11756337   Curated Info

22

Brouet A, et al. (2001) Hsp90 and caveolin are key targets for the proangiogenic nitric oxide-mediated effects of statins. Circ Res 89, 866-73
11701613   Curated Info

23

Du XL, et al. (2001) Hyperglycemia inhibits endothelial nitric oxide synthase activity by posttranslational modification at the Akt site. J Clin Invest 108, 1341-8
11696579   Curated Info

24

Chen ZP, et al. (1999) AMP-activated protein kinase phosphorylation of endothelial NO synthase. FEBS Lett 443, 285-9
10025949   Curated Info