Thr494
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Home > Phosphorylation Site Page: > Thr494  -  eNOS (rat)

Site Information
TGITRKKtFKEVANA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447885

In vivo Characterization
Methods used to characterize site in vivo:
phospho-antibody ( 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 ) , western blotting ( 1 , 3 , 4 , 6 , 7 )
Relevant cell line - cell type - tissue:
adipocyte-adipose tissue ( 5 ) , BAEC (endothelial) ( 2 ) , endothelial-aorta ( 7 ) , heart ( 8 ) , kidney ( 1 , 2 , 3 , 6 ) , penis ( 4 )

Upstream Regulation
Putative in vivo kinases:
AMPKA1 (rat) ( 8 )
Kinases, in vitro:
AMPKA1 (rat) ( 8 )
Treatments:
ACh ( 7 ) , beta-amyloid_40 ( 7 ) , cAMP_analog ( 5 ) , Go_6976 ( 7 ) , H-89 ( 5 ) , high-salt diet ( 3 ) , insulin ( 5 ) , ischemia/reperfusion ( 1 ) , leptin ( 5 ) , okadaic_acid ( 5 ) , spironolactone ( 1 ) , tautomycin ( 5 ) , U0126 ( 5 )

Downstream Regulation
Effects of modification on eNOS:
enzymatic activity, inhibited ( 1 , 8 )

References 

1

Mejía-Vilet JM, et al. (2007) Renal ischemia-reperfusion injury is prevented by the mineralocorticoid receptor blocker spironolactone. Am J Physiol Renal Physiol 293, F78-86
17376767   Curated Info

2

Mount PF, et al. (2006) Phosphorylation of neuronal and endothelial nitric oxide synthase in the kidney with high and low salt diets. Nephron Physiol 102, p36-50
16244499   Curated Info

3

Herrera M, Silva G, Garvin JL (2006) A high-salt diet dissociates NO synthase-3 expression and NO production by the thick ascending limb. Hypertension 47, 95-101
16344378   Curated Info

4

Musicki B, Kramer MF, Becker RE, Burnett AL (2005) Inactivation of phosphorylated endothelial nitric oxide synthase (Ser-1177) by O-GlcNAc in diabetes-associated erectile dysfunction. Proc Natl Acad Sci U S A 102, 11870-5
16085713   Curated Info

5

Mehebik N, et al. (2005) Leptin-induced nitric oxide production in white adipocytes is mediated through PKA and MAP kinase activation. Am J Physiol Cell Physiol 289, C379-87
15772123   Curated Info

6

Lee DL, et al. (2005) Posttranslational regulation of NO synthase activity in the renal medulla of diabetic rats. Am J Physiol Renal Physiol 288, F82-90
15383397   Curated Info

7

Gentile MT, et al. (2004) Mechanisms of soluble beta-amyloid impairment of endothelial function. J Biol Chem 279, 48135-42
15319431   Curated Info

8

Chen ZP, et al. (1999) AMP-activated protein kinase phosphorylation of endothelial NO synthase. FEBS Lett 443, 285-9
10025949   Curated Info