Ser121
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Home > Phosphorylation Site Page: > Ser121  -  MCL1 (human)

Site Information
PAADAIMsPEEELDG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 1172400

In vivo Characterization
Methods used to characterize site in vivo:
[32P] ATP in vitro ( 5 ) , [32P] bio-synthetic labeling ( 8 ) , electrophoretic mobility shift ( 8 ) , immunoprecipitation ( 1 , 4 , 6 ) , mass spectrometry ( 2 , 5 , 6 , 7 ) , mutation of modification site ( 4 , 5 , 6 , 8 ) , phospho-antibody ( 1 , 5 ) , western blotting ( 1 , 4 , 5 )
Disease tissue studied:
HER2 positive breast cancer ( 2 ) , luminal B breast cancer ( 2 ) , breast cancer, triple negative ( 2 ) , colorectal cancer ( 6 ) , colorectal carcinoma ( 6 ) , lung cancer ( 4 ) , non-small cell lung cancer ( 4 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
MKK4 (human) ( 8 ) , P38A (human) ( 8 )
Putative in vivo kinases:
CDK2 (human) ( 4 ) , JNK1 (human) ( 8 )
Kinases, in vitro:
GSK3B (human) ( 5 )
Treatments:
H2O2 ( 8 ) , nocodazole ( 7 ) , SB203580 ( 8 ) , vinblastine ( 1 )

Downstream Regulation
Effects of modification on MCL1:
molecular association, regulation ( 5 , 6 ) , protein degradation ( 5 , 6 ) , protein stabilization ( 1 ) , ubiquitination ( 5 , 6 )
Effects of modification on biological processes:
apoptosis, induced ( 6 , 8 ) , carcinogenesis, inhibited ( 6 ) , cell cycle regulation ( 6 )
Induce interaction with:
FBXW7 (human) ( 5 , 6 )

References 

1

Chu R, et al. (2016) Mitotic arrest-induced phosphorylation of Mcl-1 revisited using two-dimensional gel electrophoresis and phosphoproteomics: nine phosphorylation sites identified. Oncotarget 7, 78958-78970
27738316   Curated Info

2

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

3

Demelash A, et al. (2015) Structure-Function Analysis of the Mcl-1 Protein Identifies a Novel Senescence-regulating Domain. J Biol Chem 290, 21962-75
26205817   Curated Info

4

Choudhary GS, et al. (2015) Cyclin E/Cdk2-dependent phosphorylation of Mcl-1 determines its stability and cellular sensitivity to BH3 mimetics. Oncotarget 6, 16912-25
26219338   Curated Info

5

Inuzuka H, et al. (2011) SCF(FBW7) regulates cellular apoptosis by targeting MCL1 for ubiquitylation and destruction. Nature 471, 104-9
21368833   Curated Info

6

Wertz IE, et al. (2011) Sensitivity to antitubulin chemotherapeutics is regulated by MCL1 and FBW7. Nature 471, 110-4
21368834   Curated Info

7

Harley ME, Allan LA, Sanderson HS, Clarke PR (2010) Phosphorylation of Mcl-1 by CDK1-cyclin B1 initiates its Cdc20-dependent destruction during mitotic arrest. EMBO J 29, 2407-20
20526282   Curated Info

8

Inoshita S, et al. (2002) Phosphorylation and inactivation of myeloid cell leukemia 1 by JNK in response to oxidative stress. J Biol Chem 277, 43730-4
12223490   Curated Info