Ser569

Site Information
LDSEPAVsPLLPRKE SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 450293

In vivo Characterization
Methods used to characterize site in vivo:	[32P] bio-synthetic labeling ( 8 ) , mass spectrometry ( 1 , 2 , 3 , 4 , 5 , 6 , 7 ) , phosphopeptide mapping ( 8 )
Disease tissue studied:	anthrax infection ( 6 )
Relevant cell line - cell type - tissue:	'3T3-L1, differentiated' (adipocyte) ( 1 ) , 32Dcl3 (myeloid) [FLT3 (mouse), transfection, chimera with human FLT3-ITD mutant (corresponding to wild type P36888 ( 7 ) , 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] ( 8 ) , heart ( 4 ) , HL-1 (myocyte) [Akt1 (mouse), knockdown, stable lentiviral expression of Akt1 shRNA] ( 2 ) , HL-1 (myocyte) [Akt2 (mouse), knockdown, stable lentiviral expression of Akt2 shRNA] ( 2 ) , HL-1 (myocyte) ( 2 ) , macrophage-peritoneum ( 5 ) , spleen ( 6 ) , stromal ( 3 )

Upstream Regulation
Regulatory protein:	RIPK3 (mouse) ( 5 )
Kinases, in vitro:	CDK1 (mouse) ( 8 )

References
1	Parker BL, et al. (2015) Targeted phosphoproteomics of insulin signaling using data-independent acquisition mass spectrometry. Sci Signal 8, rs6 26060331 Curated Info
2	Reinartz M, Raupach A, Kaisers W, Gödecke A (2014) AKT1 and AKT2 induce distinct phosphorylation patterns in HL-1 cardiac myocytes. J Proteome Res 13, 4232-45 25162660 Curated Info
3	Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704 24719451 Curated Info
4	Lundby A, et al. (2013) In vivo phosphoproteomics analysis reveals the cardiac targets of β-adrenergic receptor signaling. Sci Signal 6, rs11 23737553 Curated Info
5	Wu X, et al. (2012) Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics. Mol Cell Proteomics 11, 1640-51 22942356 Curated Info
6	Manes NP, et al. (2011) Discovery of mouse spleen signaling responses to anthrax using label-free quantitative phosphoproteomics via mass spectrometry. Mol Cell Proteomics 10, M110.000927 21189417 Curated Info
7	Choudhary C, et al. (2009) Mislocalized activation of oncogenic RTKs switches downstream signaling outcomes. Mol Cell 36, 326-39 19854140 Curated Info
8	Kipreos ET, Wang JY (1990) Differential phosphorylation of c-Abl in cell cycle determined by cdc2 kinase and phosphatase activity. Science 248, 217-20 2183353 Curated Info