Ser239

Site Information
GRAPRRPsGPGPPsP SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 11546900

In vivo Characterization
Methods used to characterize site in vivo:	[32P] bio-synthetic labeling ( 3 ) , immunoassay ( 1 ) , immunoprecipitation ( 3 ) , mass spectrometry ( 2 , 3 ) , mutation of modification site ( 1 )
Relevant cell line - cell type - tissue:	HEK293T (epithelial) ( 1 , 3 ) , Jurkat (T lymphocyte) ( 2 )

Upstream Regulation
Treatments:	dopamine ( 3 )

References
1	Peeler JC, et al. (2017) Isopeptide and ester bond ubiquitination both regulate degradation of the human dopamine receptor 4. J Biol Chem 292, 21623-21630 29101232 Curated Info
2	Zhou J (2011) CST Curation Set: 12320; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
3	Spooren A, et al. (2010) Resistance of the dopamine D4 receptor to agonist-induced internalization and degradation. Cell Signal 22, 600-9 19932171 Curated Info