Ser605
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Home > Phosphorylation Site Page: > Ser605  -  NHE3 (rat)

Site Information
SLEQRRRsIRDTEDM   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 450900

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 9 ) , immunoprecipitation ( 5 , 9 ) , mutation of modification site ( 4 , 5 , 8 , 9 ) , phospho-antibody ( 1 , 2 , 3 , 4 , 6 , 7 ) , phosphoamino acid analysis ( 9 ) , phosphopeptide mapping ( 9 ) , western blotting ( 1 , 2 , 3 , 4 , 6 )
Disease tissue studied:
kidney cancer ( 6 )
Relevant cell line - cell type - tissue:
'kidney, cortex' ( 1 , 3 ) , AP-1 (fibroblast) ( 9 ) , C2BBe1 (intestinal) ( 5 ) , COS (fibroblast) ( 4 , 7 ) , kidney ( 6 , 7 ) , MDCK (epithelial) ( 2 ) , OKP (epithelial) ( 4 , 6 , 7 , 9 ) , PS120 (fibroblast) ( 4 )

Upstream Regulation
Regulatory protein:
Ezrin (rat) ( 2 )
Putative in vivo kinases:
PKACA (human) ( 1 , 3 )
Kinases, in vitro:
PKACA (rat) ( 9 )
Phosphatases, in vitro:
PPP1CA (human) ( 4 )
Treatments:
6-MB-cAMP ( 1 ) , calyculin_A ( 4 ) , cAMP_analog ( 9 ) , colforsin ( 2 , 4 , 6 , 7 ) , dopamine ( 7 ) , exenatide ( 3 ) , GLP-1 ( 3 ) , H-89 ( 3 ) , IBMX ( 2 , 4 , 6 , 7 ) , okadaic_acid ( 4 ) , P32/98 ( 3 ) , PTH ( 6 )

Downstream Regulation
Effects of modification on NHE3:
activity, inhibited ( 3 , 8 , 9 ) , intracellular localization ( 5 ) , molecular association, regulation ( 5 )
Induce interaction with:
SYT1 (human) ( 5 )

References 

1

Crajoinas RO, et al. (2014) Changes in the activity and expression of protein phosphatase-1 accompany the differential regulation of NHE3 before and after the onset of hypertension in spontaneously hypertensive rats. Acta Physiol (Oxf) 211, 395-408
24666699   Curated Info

2

Hayashi H, et al. (2013) Ezrin Is Required for the Functional Regulation of the Epithelial Sodium Proton Exchanger, NHE3. PLoS One 8, e55623
23405179   Curated Info

3

Crajoinas RO, et al. (2011) Mechanisms mediating the diuretic and natriuretic actions of the incretin hormone glucagon-like peptide-1. Am J Physiol Renal Physiol 301, F355-63
21593184   Curated Info

4

Dynia DW, Steinmetz AG, Kocinsky HS (2010) NHE3 function and phosphorylation are regulated by a calyculin A-sensitive phosphatase. Am J Physiol Renal Physiol 298, F745-53
20015946   Curated Info

5

Musch MW, et al. (2010) Cyclic AMP-mediated endocytosis of intestinal epithelial NHE3 requires binding to synaptotagmin 1. Am J Physiol Gastrointest Liver Physiol 298, G203-11
19926819   Curated Info

6

Kocinsky HS, Dynia DW, Wang T, Aronson PS (2007) NHE3 phosphorylation at serines 552 and 605 does not directly affect NHE3 activity. Am J Physiol Renal Physiol 293, F212-8
17409282   Curated Info

7

Kocinsky HS, et al. (2005) Use of phospho-specific antibodies to determine the phosphorylation of endogenous Na+/H+ exchanger NHE3 at PKA consensus sites. Am J Physiol Renal Physiol 289, F249-58
15687252   Curated Info

8

Bagorda A, et al. (2002) Reciprocal protein kinase A regulatory interactions between cystic fibrosis transmembrane conductance regulator and Na+/H+ exchanger isoform 3 in a renal polarized epithelial cell model. J Biol Chem 277, 21480-8
11937500   Curated Info

9

Zhao H, et al. (1999) Acute inhibition of Na/H exchanger NHE-3 by cAMP. Role of protein kinase a and NHE-3 phosphoserines 552 and 605. J Biol Chem 274, 3978-87
9933588   Curated Info