Ser260
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Home > Phosphorylation Site Page: > Ser260  -  RXRA (human)

Site Information
NMGLNPssPNDPVtN   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 450891

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 5 ) , immunoprecipitation ( 6 , 10 ) , mass spectrometry ( 3 ) , mutation of modification site ( 2 , 4 , 5 , 6 , 7 , 8 , 9 , 10 ) , phospho-antibody ( 6 , 8 , 9 ) , western blotting ( 6 , 9 )
Disease tissue studied:
liver cancer ( 6 , 9 ) , hepatocellular carcinoma ( 6 , 9 ) , prostate cancer ( 4 )
Relevant cell line - cell type - tissue:
COS (fibroblast) ( 8 ) , HCC (hepatic) ( 6 ) , HEK293T (epithelial) ( 6 ) , hepatocyte-liver ( 9 ) , HepG2 (hepatic) ( 7 ) , HPK1A (keratinocyte) ( 2 , 5 , 10 ) , Huh7 (hepatic) ( 6 , 9 ) , liver ( 3 ) , RWPE1 (prostate cell) ( 4 ) , RWPE2 (prostate cell) ( 4 )

Upstream Regulation
Putative in vivo kinases:
ERK1 (human) ( 10 ) , ERK2 (human) ( 8 , 9 , 10 ) , JNK1 (human) ( 7 ) , JNK2 (human) ( 7 )
Kinases, in vitro:
ERK1 (human) ( 10 ) , ERK2 (human) ( 8 , 10 )
Treatments:
1,25_dihydroxyvitamin_D ( 4 ) , culturing_of_cells ( 9 ) , PD98059 ( 5 , 9 , 10 ) , retinoic_acid ( 9 )

Downstream Regulation
Effects of modification on RXRA:
activity, inhibited ( 10 ) , intracellular localization ( 2 , 5 , 7 ) , molecular association, regulation ( 2 , 5 , 6 ) , protein stabilization ( 9 )
Effects of modification on biological processes:
apoptosis, inhibited ( 6 ) , carcinogenesis, induced ( 6 ) , cell growth, induced ( 5 , 6 ) , transcription, altered ( 8 ) , transcription, inhibited ( 4 , 5 , 6 )
Inhibit interaction with:
DNA ( 2 ) , MED1 (human) ( 5 ) , RARB (human) ( 6 ) , RXRA (human) ( 6 ) , VDR (human) ( 2 )

Disease / Diagnostics Relevance
Relevant diseases:
hepatocellular carcinoma ( 9 )

References 

1

Tang J, et al. (2018) LncRNA DANCR upregulates PI3K/AKT signaling through activating serine phosphorylation of RXRA. Cell Death Dis 9, 1167
30518934   Curated Info

2

Jusu S, Presley JF, Kremer R (2017) Phosphorylation of Human Retinoid X Receptor α at Serine 260 Impairs Its Subcellular Localization, Receptor Interaction, Nuclear Mobility, and 1α,25-Dihydroxyvitamin D3-dependent DNA Binding in Ras-transformed Keratinocytes. J Biol Chem 292, 1490-1509
27852823   Curated Info

3

Bian Y, et al. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics 96, 253-62
24275569   Curated Info

4

Zhang Z, et al. (2010) Constitutive activation of the mitogen-activated protein kinase pathway impairs vitamin D signaling in human prostate epithelial cells. J Cell Physiol 224, 433-42
20432439   Curated Info

5

Macoritto M, et al. (2008) Phosphorylation of the human retinoid X receptor alpha at serine 260 impairs coactivator(s) recruitment and induces hormone resistance to multiple ligands. J Biol Chem 283, 4943-56
18003614   Curated Info

6

Yoshimura K, et al. (2007) Phosphorylated retinoid X receptor alpha loses its heterodimeric activity with retinoic acid receptor beta. Cancer Sci 98, 1868-74
17900311   Curated Info

7

Zimmerman TL, et al. (2006) Nuclear export of retinoid X receptor alpha in response to interleukin-1beta-mediated cell signaling: roles for JNK and SER260. J Biol Chem 281, 15434-40
16551633   Curated Info

8

Dwivedi PP, et al. (2002) Role of MAP kinases in the 1,25-dihydroxyvitamin D3-induced transactivation of the rat cytochrome P450C24 (CYP24) promoter. Specific functions for ERK1/ERK2 and ERK5. J Biol Chem 277, 29643-53
12048211   Curated Info

9

Adachi S, et al. (2002) Phosphorylation of retinoid X receptor suppresses its ubiquitination in human hepatocellular carcinoma. Hepatology 35, 332-40
11826406   Curated Info

10

Solomon C, White JH, Kremer R (1999) Mitogen-activated protein kinase inhibits 1,25-dihydroxyvitamin D3-dependent signal transduction by phosphorylating human retinoid X receptor alpha. J Clin Invest 103, 1729-35
10377179   Curated Info