Ser227

Site Information
GARRRGGsASRSLPL SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 11418800

In vivo Characterization
Methods used to characterize site in vivo:	immunoprecipitation ( 2 ) , mutation of modification site ( 1 , 2 ) , phospho-antibody ( 1 ) , western blotting ( 1 )
Disease tissue studied:	bone cancer ( 2 ) , breast cancer ( 1 ) , non-small cell lung cancer ( 2 )
Relevant cell line - cell type - tissue:	MCF-7 (breast cell) ( 1 ) , NCI-H1299 (pulmonary) ( 2 ) , Saos-2 (bone cell) ( 2 )

Downstream Regulation
Effects of modification on TERT:	enzymatic activity, induced ( 3 ) , intracellular localization ( 1 , 2 )
Effects of modification on biological processes:	apoptosis, inhibited ( 2 ) , cytoskeletal reorganization ( 2 )
Induce interaction with:	KPNA1 (human) ( 1 ) , KPNA5 (human) ( 1 ) , KPNB1 (human) ( 1 )

References
1	Jeong SA, et al. (2015) Akt-mediated phosphorylation increases the binding affinity of hTERT for importin α to promote nuclear translocation. J Cell Sci 128, 2287-301 25999477 Curated Info
2	Chung J, Khadka P, Chung IK (2012) Nuclear import of hTERT requires a bipartite nuclear localization signal and Akt-mediated phosphorylation. J Cell Sci 125, 2684-97 22366458 Curated Info
3	Kang SS, Kwon T, Kwon DY, Do SI (1999) Akt protein kinase enhances human telomerase activity through phosphorylation of telomerase reverse transcriptase subunit. J Biol Chem 274, 13085-90 10224060 Curated Info