Tyr314
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Home > Phosphorylation Site Page: > Tyr314  -  PAG (mouse)

Site Information
EEEISAMySSVNKPG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 450659
Associated spectra:  1 CST

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 5 ) , mass spectrometry ( 1 , 2 , 3 ) , mutation of modification site ( 4 , 5 ) , phospho-antibody ( 4 , 5 ) , western blotting ( 4 , 5 )
Disease tissue studied:
leukemia ( 3 )
Relevant cell line - cell type - tissue:
3T3 (fibroblast) ( 5 ) , brain ( 5 ) , HEK293T (epithelial) ( 5 ) , lymphocyte-spleen ( 1 ) , RAW 264.7 (macrophage) ( 2 , 3 ) , T lymphocyte-lymph node ( 1 ) , T lymphocyte-thymus ( 4 )

Upstream Regulation
Putative in vivo kinases:
Src (rat) ( 5 )
Treatments:
anti-CD3 ( 4 ) , PP2 ( 5 ) , vanadate ( 1 )

Downstream Regulation
Effects of modification on PAG:
molecular association, regulation ( 4 )
Induce interaction with:
CSK (mouse) ( 4 )

References 

1

Reginald K, et al. (2015) Revisiting the Timing of Action of the PAG Adaptor Using Quantitative Proteomics Analysis of Primary T Cells. J Immunol 195, 5472-81
26512138   Curated Info

2

Pinto SM, et al. (2015) Quantitative phosphoproteomic analysis of IL-33-mediated signaling. Proteomics 15, 532-44
25367039   Curated Info

3

Possemato A (2009) CST Curation Set: 7166; Year: 2009; Biosample/Treatment: cell line, RAW 264.7/control; Disease: leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

4

Davidson D, Schraven B, Veillette A (2007) PAG-associated FynT regulates calcium signaling and promotes anergy in T lymphocytes. Mol Cell Biol 27, 1960-73
17210649   Curated Info

5

Kawabuchi M, et al. (2000) Transmembrane phosphoprotein Cbp regulates the activities of Src-family tyrosine kinases. Nature 404, 999-1003
10801129   Curated Info