Ser696
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Home > Phosphorylation Site Page: > Ser696  -  Raptor (human)

Site Information
EKNyALPsPAttEGG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 11315003

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 4 ) , electrophoretic mobility shift ( 3 , 10 ) , immunoprecipitation ( 7 ) , mass spectrometry ( 1 , 2 , 3 , 5 , 6 , 7 , 8 , 9 , 10 ) , mutation of modification site ( 3 , 6 , 7 , 10 ) , phospho-antibody ( 3 , 4 , 7 , 9 , 10 ) , western blotting ( 3 , 4 , 7 , 9 , 10 )
Disease tissue studied:
bone cancer ( 7 ) , HER2 positive breast cancer ( 1 ) , luminal A breast cancer ( 1 ) , luminal B breast cancer ( 1 ) , breast cancer, triple negative ( 1 ) , lung cancer ( 10 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
RHEB (human) ( 9 )
Putative in vivo kinases:
ERK1 (human) ( 7 ) , ERK2 (human) ( 7 ) , JNK1 (human) ( 3 )
Kinases, in vitro:
CDK1 (human) ( 10 ) , ERK1 (human) ( 7 ) , ERK2 (human) ( 6 , 7 ) , JNK1 (human) ( 3 )
Treatments:
EGF ( 7 ) , insulin ( 6 , 7 ) , KU-0063794 ( 4 ) , nocodazole ( 10 ) , PD184352 ( 7 ) , PD98059 ( 6 ) , phorbol_ester ( 7 ) , rapamycin ( 6 , 7 ) , siRNA ( 7 ) , sorbitol ( 3 ) , taxol ( 10 ) , U0126 ( 7 ) , wortmannin ( 6 )

Downstream Regulation
Effects of modification on Raptor:
enzymatic activity, induced ( 3 ) , phosphorylation ( 6 )

References 

1

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

2

Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71
23186163   Curated Info

3

Kwak D, et al. (2012) Osmotic Stress Regulates Mammalian Target of Rapamycin (mTOR) Complex 1 via c-Jun N-terminal Kinase (JNK)-mediated Raptor Protein Phosphorylation. J Biol Chem 287, 18398-407
22493283   Curated Info

4

Dunlop EA, et al. (2011) ULK1 inhibits mTORC1 signaling, promotes multisite Raptor phosphorylation and hinders substrate binding. Autophagy 7, 737-47
21460630   Curated Info

5

Guo A (2011) CST Curation Set: 12062; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pTXR
Curated Info

6

Langlais P, Yi Z, Mandarino LJ (2011) The Identification of Raptor as a Substrate for p44/42 MAPK. Endocrinology 152, 1264-73
21325048   Curated Info

7

Carriere A, et al. (2011) ERK1/2 phosphorylate Raptor to promote Ras-dependent activation of mTOR complex 1 (mTORC1). J Biol Chem 286, 567-77
21071439   Curated Info

8

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info

9

Foster KG, et al. (2010) Regulation of mTOR complex 1 (mTORC1) by raptor Ser863 and multisite phosphorylation. J Biol Chem 285, 80-94
19864431   Curated Info

10

Gwinn DM, Asara JM, Shaw RJ (2010) Raptor is phosphorylated by cdc2 during mitosis. PLoS One 5, e9197
20169205   Curated Info