Tyr1021
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Home > Phosphorylation Site Page: > Tyr1021  -  PDGFRB (human)

Site Information
PNEGDNDyIIPLPDP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447523

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 6 , 12 ) , [32P] bio-synthetic labeling ( 6 , 11 , 12 ) , immunoprecipitation ( 12 ) , mutation of modification site ( 3 , 5 , 7 , 9 , 11 , 12 , 13 ) , peptide sequencing ( 12 ) , phospho-antibody ( 1 , 2 , 3 , 6 ) , phosphopeptide mapping ( 6 , 11 , 12 ) , western blotting ( 1 , 2 , 3 , 7 , 9 )
Disease tissue studied:
liver cancer ( 3 , 7 )
Relevant cell line - cell type - tissue:
3T3 (fibroblast) ( 3 ) , HEK293T (epithelial) ( 5 ) , HepG2 (hepatic) ( 3 , 7 ) , neuron-'brain, cerebral cortex' ( 1 ) , neuron-'brain, hippocampus' ( 1 ) , PAE (endothelial) ( 6 , 12 , 13 ) , TRMP (renal) ( 9 ) , vascular smooth muscle cell ('muscle, smooth') ( 2 )

Upstream Regulation
Regulatory protein:
PIK3C2A (human) ( 7 ) , RAC1 (human) ( 7 )
Putative in vivo kinases:
PDGFRB (human) ( 6 , 12 )
Kinases, in vitro:
PDGFRB (human) ( 8 , 11 , 12 )
Putative upstream phosphatases:
ACP1 (human) ( 3 ) , PTPRJ (human) ( 6 )
Phosphatases, in vitro:
PTP1B (human) ( 8 ) , PTPRJ (human) ( 4 , 6 )
Treatments:
AG1296 ( 2 ) , AG17 ( 1 ) , angiotensin ( 2 ) , candesartan ( 2 ) , LY294002 ( 7 ) , PD168077 ( 1 ) , PDGF ( 1 , 2 , 3 , 6 , 7 , 12 ) , PP2 ( 2 ) , quinpirole ( 1 ) , raclopride ( 1 ) , wortmannin ( 7 )

Downstream Regulation
Effects of modification on PDGFRB:
activity, induced ( 13 ) , molecular association, regulation ( 10 , 11 , 12 ) , phosphorylation ( 5 )
Induce interaction with:
PLCG1 (human) ( 11 , 12 ) , PLCG1 (rat) ( 10 )

References 

1

Beazely MA, et al. (2006) D2-class dopamine receptor inhibition of NMDA currents in prefrontal cortical neurons is platelet-derived growth factor receptor-dependent. J Neurochem 98, 1657-63
16879713   Curated Info

2

Gao BB, Hansen H, Chen HC, Feener EP (2006) Angiotensin II stimulates phosphorylation of an ectodomain-truncated platelet-derived growth factor receptor-beta and its binding to class IA PI3K in vascular smooth muscle cells. Biochem J 397, 337-44
16569213   Curated Info

3

Chiarugi P, et al. (2002) Insight into the role of low molecular weight phosphotyrosine phosphatase (LMW-PTP) on platelet-derived growth factor receptor (PDGF-r) signaling. LMW-PTP controls PDGF-r kinase activity through TYR-857 dephosphorylation. J Biol Chem 277, 37331-8
12149261   Curated Info

4

Persson C, Engström U, Mowbray SL, Ostman A (2002) Primary sequence determinants responsible for site-selective dephosphorylation of the PDGF beta-receptor by the receptor-like protein tyrosine phosphatase DEP-1. FEBS Lett 517, 27-31
12062403   Curated Info

5

Fang X, et al. (2002) Convergence of multiple signaling cascades at glycogen synthase kinase 3: Edg receptor-mediated phosphorylation and inactivation by lysophosphatidic acid through a protein kinase C-dependent intracellular pathway. Mol Cell Biol 22, 2099-110
11884598   Curated Info

6

Kovalenko M, et al. (2000) Site-selective dephosphorylation of the platelet-derived growth factor beta-receptor by the receptor-like protein-tyrosine phosphatase DEP-1. J Biol Chem 275, 16219-26
10821867   Curated Info

7

Bae YS, et al. (2000) Platelet-derived growth factor-induced H(2)O(2) production requires the activation of phosphatidylinositol 3-kinase. J Biol Chem 275, 10527-31
10744745   Curated Info

8

Klinghoffer RA, Kazlauskas A (1995) Identification of a putative Syp substrate, the PDGF beta receptor. J Biol Chem 270, 22208-17
7545675   Curated Info

9

Franke TF, et al. (1995) The protein kinase encoded by the Akt proto-oncogene is a target of the PDGF-activated phosphatidylinositol 3-kinase. Cell 81, 727-36
7774014   Curated Info

10

Larose L, Gish G, Pawson T (1995) Construction of an SH2 domain-binding site with mixed specificity. J Biol Chem 270, 3858-62
7876130   Curated Info

11

Valius M, Bazenet C, Kazlauskas A (1993) Tyrosines 1021 and 1009 are phosphorylation sites in the carboxy terminus of the platelet-derived growth factor receptor beta subunit and are required for binding of phospholipase C gamma and a 64-kilodalton protein, respectively. Mol Cell Biol 13, 133-43
7678051   Curated Info

12

Rönnstrand L, et al. (1992) Identification of two C-terminal autophosphorylation sites in the PDGF beta-receptor: involvement in the interaction with phospholipase C-gamma. EMBO J 11, 3911-9
1396585   Curated Info

13

Ridefelt P, Siegbahn A Tyr1009 and Tyr1021 in the platelet-derived growth factor beta-receptor mediate agonist triggered calcium signalling. Anticancer Res 18, 1819-25
9673410   Curated Info