Tyr1009
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Home > Phosphorylation Site Page: > Tyr1009  -  PDGFRB (human)

Site Information
LDTSSVLyTAVQPNE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447522

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 6 , 13 ) , [32P] bio-synthetic labeling ( 6 , 8 , 11 , 12 , 13 ) , immunoprecipitation ( 13 ) , mutation of modification site ( 1 , 2 , 4 , 5 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 ) , peptide sequencing ( 8 , 11 , 13 ) , phospho-antibody ( 6 , 8 ) , phosphoamino acid analysis ( 8 ) , phosphopeptide mapping ( 6 , 8 , 11 , 12 , 13 ) , western blotting ( 7 , 10 )
Disease tissue studied:
liver cancer ( 7 , 9 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 2 ) , HepG2 (hepatic) ( 7 , 9 ) , PAE (endothelial) ( 1 , 4 , 6 , 8 , 11 , 13 , 14 ) , Rat1 (fibroblast) ( 5 ) , TRMP (renal) ( 10 )

Upstream Regulation
Regulatory protein:
PIK3C2A (human) ( 7 ) , RAC1 (human) ( 7 )
Putative in vivo kinases:
PDGFRB (human) ( 6 , 8 , 13 )
Kinases, in vitro:
PDGFRB (human) ( 9 , 12 , 13 )
Putative upstream phosphatases:
PTPRJ (human) ( 6 )
Phosphatases, in vitro:
PTP1B (human) ( 9 ) , PTPRJ (human) ( 6 )
Treatments:
LY294002 ( 7 ) , PDGF ( 6 , 7 , 8 , 13 ) , wortmannin ( 7 )

Downstream Regulation
Effects of modification on PDGFRB:
activity, induced ( 14 ) , molecular association, regulation ( 2 , 3 , 4 , 5 , 8 , 9 , 11 , 13 ) , phosphorylation ( 5 , 9 )
Effects of modification on biological processes:
cell growth, altered ( 3 )
Induce interaction with:
CBL (human) ( 3 ) , PLCG1 (human) ( 13 ) , SH3KBP1 (human) ( 3 ) , SHP-2 (human) ( 2 , 4 , 5 , 8 , 11 )
Inhibit interaction with:
PIK3R1 (human) ( 9 ) , RASA1 (human) ( 9 )

References 

1

Lennartsson J, et al. (2010) Erk 5 is necessary for sustained PDGF-induced Akt phosphorylation and inhibition of apoptosis. Cell Signal 22, 955-60
20138986   Curated Info

2

Mitchell D, et al. (2007) The Lipoxin A4 receptor is coupled to SHP-2 activation: implications for regulation of receptor tyrosine kinases. J Biol Chem 282, 15606-18
17403678   Curated Info

3

Szymkiewicz I, et al. (2002) CIN85 participates in Cbl-b-mediated down-regulation of receptor tyrosine kinases. J Biol Chem 277, 39666-72
12177062   Curated Info

4

Ekman S, et al. (2002) SHP-2 is involved in heterodimer specific loss of phosphorylation of Tyr771 in the PDGF beta-receptor. Oncogene 21, 1870-5
11896619   Curated Info

5

Meng TC, Fukada T, Tonks NK (2002) Reversible oxidation and inactivation of protein tyrosine phosphatases in vivo. Mol Cell 9, 387-99
11864611   Curated Info

6

Kovalenko M, et al. (2000) Site-selective dephosphorylation of the platelet-derived growth factor beta-receptor by the receptor-like protein-tyrosine phosphatase DEP-1. J Biol Chem 275, 16219-26
10821867   Curated Info

7

Bae YS, et al. (2000) Platelet-derived growth factor-induced H(2)O(2) production requires the activation of phosphatidylinositol 3-kinase. J Biol Chem 275, 10527-31
10744745   Curated Info

8

Rönnstrand L, et al. (1999) SHP-2 binds to Tyr763 and Tyr1009 in the PDGF beta-receptor and mediates PDGF-induced activation of the Ras/MAP kinase pathway and chemotaxis. Oncogene 18, 3696-702
10391677   Curated Info

9

Klinghoffer RA, Kazlauskas A (1995) Identification of a putative Syp substrate, the PDGF beta receptor. J Biol Chem 270, 22208-17
7545675   Curated Info

10

Franke TF, et al. (1995) The protein kinase encoded by the Akt proto-oncogene is a target of the PDGF-activated phosphatidylinositol 3-kinase. Cell 81, 727-36
7774014   Curated Info

11

Arvidsson AK, et al. (1994) Tyr-716 in the platelet-derived growth factor beta-receptor kinase insert is involved in GRB2 binding and Ras activation. Mol Cell Biol 14, 6715-26
7935391   Curated Info

12

Valius M, Bazenet C, Kazlauskas A (1993) Tyrosines 1021 and 1009 are phosphorylation sites in the carboxy terminus of the platelet-derived growth factor receptor beta subunit and are required for binding of phospholipase C gamma and a 64-kilodalton protein, respectively. Mol Cell Biol 13, 133-43
7678051   Curated Info

13

Rönnstrand L, et al. (1992) Identification of two C-terminal autophosphorylation sites in the PDGF beta-receptor: involvement in the interaction with phospholipase C-gamma. EMBO J 11, 3911-9
1396585   Curated Info

14

Ridefelt P, Siegbahn A Tyr1009 and Tyr1021 in the platelet-derived growth factor beta-receptor mediate agonist triggered calcium signalling. Anticancer Res 18, 1819-25
9673410   Curated Info