Ser81
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Home > Phosphorylation Site Page: > Ser81  -  TDP1 (human)

Site Information
PKRQKsGsQEDLGWC   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 11279401

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 4 ) , mass spectrometry ( 1 , 4 ) , mutation of modification site ( 2 ) , phospho-antibody ( 3 , 4 ) , western blotting ( 2 , 3 , 4 )
Disease tissue studied:
HER2 positive breast cancer ( 1 ) , luminal A breast cancer ( 1 ) , luminal B breast cancer ( 1 ) , breast cancer, triple negative ( 1 ) , colorectal cancer ( 3 ) , colorectal carcinoma ( 3 ) , lung cancer ( 2 ) , non-small cell lung cancer ( 2 ) , non-small cell lung adenocarcinoma ( 2 )
Relevant cell line - cell type - tissue:
A549 (pulmonary) ( 2 ) , breast ( 1 ) , E.coli (bacterial) ( 2 ) , HCT116 (intestinal) ( 3 ) , HeLa (cervical) ( 4 ) , LCL (lymphoblastoid) ( 2 ) , MEF (fibroblast) ( 2 , 3 )

Upstream Regulation
Putative in vivo kinases:
ATM (human) ( 3 , 4 ) , DNAPK (human) ( 3 )
Kinases, in vitro:
ATM (human) ( 3 ) , DNAPK (human) ( 3 )
Treatments:
aphidicolin ( 3 ) , camptothecin ( 3 ) , DRB ( 3 ) , hydroxyurea ( 4 ) , ionizing_radiation ( 3 , 4 ) , KU-55933 ( 3 ) , NU7441 ( 3 )

Downstream Regulation
Effects of modification on TDP1:
intracellular localization ( 3 ) , molecular association, regulation ( 2 , 3 ) , protein stabilization ( 2 , 3 )
Induce interaction with:
LIG3 (human) ( 2 ) , XRCC1 (human) ( 3 )

References 

1

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

2

Chiang SC, Carroll J, El-Khamisy SF (2010) TDP1 serine 81 promotes interaction with DNA ligase IIIalpha and facilitates cell survival following DNA damage. Cell Cycle 9, 588-595
20009512   Curated Info

3

Das BB, et al. (2009) Optimal function of the DNA repair enzyme TDP1 requires its phosphorylation by ATM and/or DNA-PK. EMBO J 28, 3667-80
19851285   Curated Info

4

Mu JJ, et al. (2007) A proteomic analysis of ataxia telangiectasia-mutated (ATM)/ATM-Rad3-related (ATR) substrates identifies the ubiquitin-proteasome system as a regulator for DNA damage checkpoints. J Biol Chem 282, 17330-4
17478428   Curated Info