Ser262

Site Information
SPSKDCGsPKYAYFN SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 450694

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 7 , 9 , 10 ) , microscopy-colocalization with upstream kinase ( 5 ) , mutation of modification site ( 3 , 8 ) , phospho-antibody ( 1 , 2 , 4 , 5 , 6 , 11 ) , western blotting ( 1 , 2 , 4 , 6 )
Relevant cell line - cell type - tissue:	'heart, ventricle' ( 6 ) , brain ( 3 , 7 ) , heart ( 1 , 10 ) , MEF (fibroblast) [p53 (mouse), homozygous knockout] ( 9 ) , myocyte-heart ( 2 , 6 ) , oocyte ( 8 ) , ovary ( 5 , 8 , 11 ) , TM4 (sertoli) ( 4 )

Upstream Regulation
Treatments:	2-APB ( 6 ) , ATP ( 6 ) , benzo(a)pyrene ( 4 ) , LH ( 11 ) , U0124 ( 11 ) , U0126 ( 11 ) , XeC ( 6 )

References
1	Solan JL, Marquez-Rosado L, Lampe PD (2019) Cx43 phosphorylation mediated effects on ERK and Akt protect against ischemia reperfusion injury and alter stability of stress-inducible protein NDRG1. J Biol Chem 31189653 Curated Info
2	Sun Z, et al. (2019) Connexin 43-serine 282 modulates serine 279 phosphorylation in cardiomyocytes. Biochem Biophys Res Commun 513, 567-572 30981509 Curated Info
3	Freitas-Andrade M, et al. (2019) Targeting MAPK phosphorylation of Connexin43 provides neuroprotection in stroke. J Exp Med 216, 916-935 30872361 Curated Info
4	Ji X, et al. (2018) Benzo[a]pyrene-decreased gap junctional intercellular communication via calcium/calmodulin signaling increases apoptosis in TM4 cells. J Appl Toxicol 29578247 Curated Info
5	Norris RP, Baena V, Terasaki M (2017) Localization of phosphorylated connexin 43 using serial section immunogold electron microscopy. J Cell Sci 130, 1333-1340 28202692 Curated Info
6	Kang M, et al. (2014) Cx43 phosphorylation on S279/282 and intercellular communication are regulated by IP3/IP3 receptor signaling. Cell Commun Signal 12, 58 25262337 Curated Info
7	Trinidad JC, et al. (2012) Global identification and characterization of both O-GlcNAcylation and phosphorylation at the murine synapse. Mol Cell Proteomics 11, 215-29 22645316 Curated Info
8	Dyce PW, Norris RP, Lampe PD, Kidder GM (2012) Phosphorylation of Serine Residues in the C-terminal Cytoplasmic Tail of Connexin43 Regulates Proliferation of Ovarian Granulosa Cells. J Membr Biol 245, 291-301 22729691 Curated Info
9	Hsu PP, et al. (2011) The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Science 332, 1317-22 21659604 Curated Info
10	Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89 21183079 Curated Info
11	Norris RP, et al. (2008) Luteinizing hormone causes MAP kinase-dependent phosphorylation and closure of connexin 43 gap junctions in mouse ovarian follicles: one of two paths to meiotic resumption. Development 135, 3229-38 18776144 Curated Info