Ser262
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Home > Phosphorylation Site Page: > Ser262  -  GJA1 (human)

Site Information
sPAkDCGsQkyAyFN   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 450694

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 3 ) , mass spectrometry ( 8 , 9 , 10 , 11 , 12 ) , mutation of modification site ( 3 , 6 , 13 , 14 ) , phospho-antibody ( 2 , 3 , 4 , 13 , 14 , 15 ) , western blotting ( 2 , 3 , 4 , 6 , 13 )
Disease tissue studied:
atrial fibrillation ( 12 ) , ventricular tachycardia ( 8 , 9 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 14 ) , 3T3 (fibroblast) ( 6 ) , CHO (fibroblast) ( 13 ) , E14tg2a ('stem, embryonic') ( 4 ) , H9c2 (myoblast) ( 3 ) , heart ( 8 , 9 , 11 , 12 ) , HeLa (cervical) ( 13 ) , HES-3 ('stem, embryonic') ( 10 ) , HMEC (endothelial) ( 2 ) , IMR32 (neural crest) ( 15 ) , MDCK (epithelial) ( 13 )

Upstream Regulation
Regulatory protein:
HSP70 (human) ( 2 ) , PKCE (human) ( 3 )
Treatments:
colforsin ( 15 ) , EGF ( 4 ) , hypoxia ( 3 ) , phorbol_ester ( 3 , 13 , 14 , 15 ) , PKC_inhibitor ( 3 )

Downstream Regulation
Effects of modification on GJA1:
intracellular localization ( 15 ) , molecular association, regulation ( 3 , 4 , 6 )
Effects of modification on biological processes:
apoptosis, inhibited ( 3 ) , cell growth, altered ( 14 ) , endocytosis, induced ( 4 )
Induce interaction with:
CLTA (human) ( 4 ) , KCNJ8 (human) ( 3 )
Inhibit interaction with:
KCNJ8 (human) ( 6 )

References 

1

Boeldt DS, et al. (2015) Phosphorylation of Ser-279/282 and Tyr-265 positions on Cx43 as possible mediators of VEGF-165 inhibition of pregnancy-adapted Ca2+ burst function in ovine uterine artery endothelial cells. Mol Cell Endocrinol 412, 73-84
26033246   Curated Info

2

Thuringer D, et al. (2015) Oncogenic extracellular HSP70 disrupts the gap-junctional coupling between capillary cells. Oncotarget 6, 10267-83
25868858   Curated Info

3

Waza AA, Andrabi K, Hussain MU (2014) Protein kinase C (PKC) mediated interaction between conexin43 (Cx43) and K(+)(ATP) channel subunit (Kir6.1) in cardiomyocyte mitochondria: Implications in cytoprotection against hypoxia induced cell apoptosis. Cell Signal 26, 1909-17
24815185   Curated Info

4

Fong JT, Nimlamool W, Falk MM (2014) EGF induces efficient Cx43 gap junction endocytosis in mouse embryonic stem cell colonies via phosphorylation of Ser262, Ser279/282, and Ser368. FEBS Lett 588, 836-44
24492000   Curated Info

5

Grosely R, et al. (2013) Effects of Phosphorylation on the Structure and Backbone Dynamics of the Intrinsically Disordered Connexin43 C-terminal Domain. J Biol Chem 288, 24857-70
23828237   Curated Info

6

Ahmad Waza A, Andrabi K, Ul Hussain M (2012) Adenosine-triphosphate-sensitive K+ channel (Kir6.1): a novel phosphospecific interaction partner of connexin 43 (Cx43). Exp Cell Res 318, 2559-66
22960107   Curated Info

7

Joshi CN, et al. (2012) Control of vascular smooth muscle cell growth by connexin 43. Front Physiol 3, 220
22737133   Curated Info

8

Li Y (2011) CST Curation Set: 10516; Year: 2011; Biosample/Treatment: tissue, heart/untreated; Disease: ventricular tachycardia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

9

Li Y (2011) CST Curation Set: 10517; Year: 2011; Biosample/Treatment: tissue, heart/untreated; Disease: ventricular tachycardia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

10

Ding VM, et al. (2011) Tyrosine phosphorylation profiling in fgf-2 stimulated human embryonic stem cells. PLoS One 6, e17538
21437283   Curated Info

11

Stokes M (2008) CST Curation Set: 4143; Year: 2008; Biosample/Treatment: tissue, heart/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

12

Stokes M (2008) CST Curation Set: 4145; Year: 2008; Biosample/Treatment: tissue, heart/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

13

Solan JL, Lampe PD (2007) Key connexin 43 phosphorylation events regulate the gap junction life cycle. J Membr Biol 217, 35-41
17629739   Curated Info

14

Dang X, Jeyaraman M, Kardami E (2006) Regulation of connexin-43-mediated growth inhibition by a phosphorylatable amino-acid is independent of gap junction-forming ability. Mol Cell Biochem 289, 201-7
16718370   Curated Info

15

Arnold JM, Phipps MW, Chen J, Phipps J (2005) Cellular sublocalization of Cx43 and the establishment of functional coupling in IMR-32 neuroblastoma cells. Mol Carcinog 42, 159-69
15605363   Curated Info

16

Doble BW, et al. (2004) Phosphorylation of serine 262 in the gap junction protein connexin-43 regulates DNA synthesis in cell-cell contact forming cardiomyocytes. J Cell Sci 117, 507-14
14702389   Curated Info