Ser262
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Home > Phosphorylation Site Page: > Ser262  -  GJA1 (rat)

Site Information
sPskDCGsPkyAyFN   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 450694

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 10 ) , mutation of modification site ( 6 , 7 , 8 , 9 , 11 ) , phospho-antibody ( 1 , 2 , 3 , 4 , 5 , 6 , 8 , 9 , 11 , 12 ) , western blotting ( 1 , 2 , 3 , 4 , 5 , 6 , 8 , 9 , 12 )
Relevant cell line - cell type - tissue:
'heart, embryo' ( 9 ) , 'heart, ventricle' ( 2 , 4 ) , E.coli (bacterial) ( 8 ) , H9c2 (myoblast) ( 8 ) , heart ( 3 , 5 , 10 , 12 ) , HUVEC (endothelial) ( 1 ) , myocyte-heart ( 3 , 4 , 6 , 11 ) , oocyte ( 7 )

Upstream Regulation
Regulatory protein:
GJA1 (human) ( 3 ) , IP3R1 (rat) ( 4 ) , TGFBR2 (rat) ( 6 )
Putative in vivo kinases:
PKCA (rat) ( 11 ) , PKCE (rat) ( 5 )
Treatments:
2-APB ( 4 ) , albumin ( 1 ) , epsilonV1-2 ( 5 ) , FGF2 ( 5 , 6 , 9 , 11 , 12 ) , ischemia ( 9 ) , phorbol_ester ( 5 , 11 ) , SB431542 ( 6 ) , TGF-beta ( 6 )

Downstream Regulation
Effects of modification on biological processes:
cell cycle regulation ( 6 ) , cell growth, altered ( 11 ) , cell growth, induced ( 6 ) , signaling pathway regulation ( 6 )

Disease / Diagnostics Relevance
Relevant diseases:
hypertrophic cardiomyopathy ( 2 )

References 

1

Huang Y, et al. (2022) Glycated serum albumin decreases connexin 43 phosphorylation in the corpus cavernosum. Transl Androl Urol 11, 1486-1494
36507487   Curated Info

2

Fu ZP, et al. (2021) Connexin 43 hyper-phosphorylation at serine 282 triggers apoptosis in rat cardiomyocytes via activation of mitochondrial apoptotic pathway. Acta Pharmacol Sin
34931018   Curated Info

3

Yang Y, et al. (2019) Connexin43 dephosphorylation at serine 282 is associated with connexin43-mediated cardiomyocyte apoptosis. Cell Death Differ
30770876   Curated Info

4

Kang M, et al. (2014) Cx43 phosphorylation on S279/282 and intercellular communication are regulated by IP3/IP3 receptor signaling. Cell Commun Signal 12, 58
25262337   Curated Info

5

Srisakuldee W, et al. (2014) The FGF-2-triggered protection of cardiac subsarcolemmal mitochondria from calcium overload is mitochondrial connexin 43-dependent. Cardiovasc Res 103, 72-80
24654232   Curated Info

6

Jeyaraman MM, Fandrich RR, Kardami E (2013) Together and apart: inhibition of DNA synthesis by connexin-43 and its relationship to transforming growth factor β. Front Pharmacol 4, 90
23882217   Curated Info

7

Mitra SS, Xu J, Nicholson BJ (2012) Coregulation of Multiple Signaling Mechanisms in pp60v-Src-Induced Closure of Cx43 Gap Junction Channels. J Membr Biol 245, 495-506
22965738   Curated Info

8

Xu HJ, et al. (2012) S262A mutation abolishes protective effects of connexin 43 against hypothermic preservation-induced injury in cardiomyocytes. J Heart Lung Transplant 31, 663-9
22608771   Curated Info

9

Srisakuldee W, et al. (2009) Phosphorylation of connexin-43 at serine 262 promotes a cardiac injury-resistant state. Cardiovasc Res 83, 672-81
19423616   Curated Info

10

Axelsen LN, et al. (2006) Identification of ischemia-regulated phosphorylation sites in connexin43: A possible target for the antiarrhythmic peptide analogue rotigaptide (ZP123). J Mol Cell Cardiol 40, 790-8
16678851   Curated Info

11

Doble BW, et al. (2004) Phosphorylation of serine 262 in the gap junction protein connexin-43 regulates DNA synthesis in cell-cell contact forming cardiomyocytes. J Cell Sci 117, 507-14
14702389   Curated Info

12

Srisakuldee W, et al. Administration of FGF-2 to the heart stimulates connexin-43 phosphorylation at protein kinase C target sites. Cell Commun Adhes 13, 13-9
16613776   Curated Info

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