Thr258
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Home > Phosphorylation Site Page: > Thr258  -  PHB (human)

Site Information
LSRSRNItyLPAGQs   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 11139253

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 4 ) , mass spectrometry ( 5 ) , mutation of modification site ( 2 , 4 ) , phospho-antibody ( 1 , 2 , 3 , 4 ) , western blotting ( 1 , 2 , 3 , 4 )
Disease tissue studied:
bladder cancer ( 3 ) , breast cancer ( 4 ) , cervical cancer ( 4 ) , cervical squamous cell carcinoma ( 4 ) , colorectal cancer ( 1 ) , colorectal carcinoma ( 1 ) , lung cancer ( 2 , 4 ) , non-small cell lung cancer ( 2 , 4 ) , non-small cell lung adenocarcinoma ( 2 ) , prostate cancer ( 4 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
ERas (human) ( 4 )
Putative in vivo kinases:
Akt1 (human) ( 3 , 4 )
Treatments:
EGF ( 2 ) , HGF ( 2 ) , IGF-1 ( 2 ) , PGE2 ( 2 )

Downstream Regulation
Effects of modification on PHB:
intracellular localization ( 1 , 3 , 4 ) , molecular association, regulation ( 1 , 3 , 4 ) , phosphorylation ( 4 ) , protein stabilization ( 1 )
Effects of modification on biological processes:
apoptosis, inhibited ( 1 ) , carcinogenesis, induced ( 1 , 4 ) , cell growth, induced ( 3 ) , cell motility, induced ( 2 , 4 ) , signaling pathway regulation ( 1 , 4 ) , transcription, inhibited ( 1 )
Induce interaction with:
RAF1 (human) ( 4 )
Inhibit interaction with:
DNA ( 1 ) , ER-alpha (human) ( 3 )

Disease / Diagnostics Relevance
Relevant diseases:
cervical adenocarcinoma ( 4 )

References 

1

Jackson DN, et al. (2020) The synthetic small molecule FL3 combats intestinal tumorigenesis via Axin1-mediated inhibition of Wnt/beta-catenin signaling. Cancer Res
32665357   Curated Info

2

Ho MY, Liang CM, Liang SM (2015) MIG-7 and phosphorylated prohibitin coordinately regulate lung cancer invasion/metastasis. Oncotarget 6, 381-93
25575814   Curated Info

3

Jiang L, et al. (2015) Akt phosphorylates Prohibitin 1 to mediate its mitochondrial localization and promote proliferation of bladder cancer cells. Cell Death Dis 6, e1660
25719244   Curated Info

4

Chiu CF, et al. (2013) Raf activation by Ras and promotion of cellular metastasis require phosphorylation of prohibitin in the raft domain of the plasma membrane. Oncogene 32, 777-87
22410782   Curated Info

5

Zhou J (2009) CST Curation Set: 7637; Year: 2009; Biosample/Treatment: cell line, HeLa/untreated; Disease: cervical adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS*/T*) (23C8D2) Rabbit mAb Cat#: 10001, PTMScan(R) Phospho-Akt Substrate Motif (RXRXXS*/T*) Immunoaffinity Beads Cat#: 1979
Curated Info