Tyr433
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Home > Phosphorylation Site Page: > Tyr433  -  SQSTM1 (human)

Site Information
AALDtIQyskHPPPL   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 11112748

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 2 , 3 , 4 ) , mutation of modification site ( 1 ) , phospho-antibody ( 1 ) , western blotting ( 1 )
Disease tissue studied:
liver cancer ( 4 ) , hepatocellular carcinoma ( 4 ) , lung cancer ( 1 , 2 ) , non-small cell lung cancer ( 1 , 2 ) , non-small cell lung adenocarcinoma ( 1 , 2 )
Relevant cell line - cell type - tissue:
A549 (pulmonary) ( 1 ) , HCC78 (pulmonary) ( 2 ) , HEK293T (epithelial) ( 1 ) , hepatocyte-liver ( 4 ) , MDA-MB-231 (breast cell) [Syk (human), transfection, Tet-reponsive Syk-inducible system] ( 3 ) , NCI-H1975 (pulmonary) ( 1 )

Upstream Regulation
Putative in vivo kinases:
EGFR (human) ( 1 )
Treatments:
EGF ( 1 ) , siRNA ( 1 )

Downstream Regulation
Effects of modification on SQSTM1:
activity, inhibited ( 1 ) , molecular association, regulation ( 1 ) , protein stabilization ( 1 )
Effects of modification on biological processes:
autophagy, inhibited ( 1 ) , carcinogenesis, induced ( 1 )
Induce interaction with:
SQSTM1 (human) ( 1 )

References 

1

Yu JJ, et al. (2020) Disruption of the EGFR-SQSTM1 interaction by a stapled peptide suppresses lung cancer via activating autophagy and inhibiting EGFR signaling. Cancer Lett 474, 23-35
31931029   Curated Info

2

Possemato A (2011) CST Curation Set: 11922; Year: 2011; Biosample/Treatment: cell line, HCC78/untreated; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

3

Iliuk AB, et al. (2010) In-depth analyses of kinase-dependent tyrosine phosphoproteomes based on metal ion-functionalized soluble nanopolymers. Mol Cell Proteomics 9, 2162-72
20562096   Curated Info

4

Gu T (2009) CST Curation Set: 7544; Year: 2009; Biosample/Treatment: liver, hepatocyte/untreated; Disease: liver cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

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