Tyr314
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Home > Phosphorylation Site Page: > Tyr314  -  PAG (rat)

Site Information
EEEISAMysSVNKPG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 450659

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 13 ) , immunoprecipitation ( 11 ) , mass spectrometry ( 1 , 2 , 4 , 5 , 6 , 7 , 8 , 9 , 10 ) , microscopy-colocalization with upstream kinase ( 3 ) , mutation of modification site ( 3 , 11 , 13 ) , phospho-antibody ( 12 , 13 ) , phosphopeptide mapping ( 12 ) , western blotting ( 3 , 13 )
Disease tissue studied:
adrenal cancer ( 7 , 8 , 9 ) , pheochromocytoma ( 7 , 8 , 9 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
LYN (mouse) ( 3 ) , Src (rat) ( 13 )
Kinases, in vitro:
Src (rat) ( 13 )
Treatments:
Epo ( 11 )

Downstream Regulation
Effects of modification on PAG:
intracellular localization ( 3 ) , molecular association, regulation ( 3 , 11 , 12 , 13 )
Effects of modification on biological processes:
cell adhesion, altered ( 3 ) , cell cycle regulation ( 13 ) , cell differentiation, altered ( 11 ) , cytoskeletal reorganization ( 3 )
Induce interaction with:
CSK (human) ( 11 ) , CSK (rat) ( 12 , 13 ) , CTK (human) ( 11 ) , SOCS1 (human) ( 11 )

References 

1

Hu Y (2009) CST Curation Set: 8261; Year: 2009; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

2

Hu Y (2009) CST Curation Set: 8260; Year: 2009; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

3

Ingley E (2009) Csk-binding protein can regulate Lyn signals controlling cell morphology. Int J Biochem Cell Biol 41, 1332-43
19124084   Curated Info

4

Guo A (2009) CST Curation Set: 5865; Year: 2009; Biosample/Treatment: tissue, brain/seizure; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

5

Guo A (2008) CST Curation Set: 5216; Year: 2008; Biosample/Treatment: tissue, brain/mock surgery; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

6

Guo A (2008) CST Curation Set: 5217; Year: 2008; Biosample/Treatment: tissue, brain/mock surgery; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

7

Stokes M (2008) CST Curation Set: 4008; Year: 2008; Biosample/Treatment: cell line, PC-12/untreated; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

8

Stokes M (2008) CST Curation Set: 4009; Year: 2008; Biosample/Treatment: cell line, PC-12/EGF; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

9

Stokes M (2008) CST Curation Set: 4013; Year: 2008; Biosample/Treatment: cell line, PC-12/PACAP; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

10

Guo A (2008) CST Curation Set: 3880; Year: 2008; Biosample/Treatment: cell line, PC-12/NGF; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

11

Ingley E, et al. (2006) Csk-binding protein mediates sequential enzymatic down-regulation and degradation of Lyn in erythropoietin-stimulated cells. J Biol Chem 281, 31920-9
16920712   Curated Info

12

Takeuchi S, et al. (2000) Transmembrane phosphoprotein Cbp positively regulates the activity of the carboxyl-terminal Src kinase, Csk. J Biol Chem 275, 29183-6
10918051   Curated Info

13

Kawabuchi M, et al. (2000) Transmembrane phosphoprotein Cbp regulates the activities of Src-family tyrosine kinases. Nature 404, 999-1003
10801129   Curated Info