Ser395

Site Information
PSFTRHPsLIKLVKS SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 10994497

In vivo Characterization
Methods used to characterize site in vivo:	[32P] ATP in vitro ( 1 ) , flow cytometry ( 1 ) , immunoprecipitation ( 1 ) , mass spectrometry ( 1 , 3 ) , mutation of modification site ( 1 ) , phospho-antibody ( 1 ) , western blotting ( 1 )
Disease tissue studied:	bone cancer ( 1 ) , testicular cancer ( 3 )
Relevant cell line - cell type - tissue:	F9 (testicular) ( 3 ) , HEK293T (epithelial) ( 1 ) , HeLa (cervical) ( 1 ) , U2OS (bone cell) ( 1 )

Downstream Regulation
Effects of modification on E2F8:	molecular association, regulation ( 1 )
Effects of modification on biological processes:	apoptosis, inhibited ( 1 ) , cell cycle regulation ( 1 ) , transcription, induced ( 1 )
Induce interaction with:	14-3-3 zeta (human) ( 1 )

References
1	Yuan R, et al. (2018) Chk1 and 14-3-3 proteins inhibit atypical E2Fs to prevent a permanent cell cycle arrest. EMBO J 29363506 Curated Info
2	Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704 24719451 Curated Info
3	Zhou J (2010) CST Curation Set: 9677; Year: 2010; Biosample/Treatment: cell line, F9/untreated; Disease: testicular cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb Cat#: 9624, PTMScan(R) Phospho-PKA Substrate Motif (K/RK/RXS/T) Immunoaffinity Beads Cat#: 1984 Curated Info