Ser226

Site Information
NEENRAKsVLKKLRG SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 10827359

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 2 , 4 , 5 , 6 )
Disease tissue studied:	lung cancer ( 5 )
Relevant cell line - cell type - tissue:	CL1-0 (pulmonary) ( 5 ) , CL1-1 (pulmonary) ( 5 ) , CL1-2 (pulmonary) ( 5 ) , CL1-5 (pulmonary) ( 5 ) , HUES-9 ('stem, embryonic') ( 4 ) , Jurkat (T lymphocyte) ( 6 ) , SH-SY5Y (neural crest) [LRRK2 (human), transfection, over-expression of LRRK2(G2019S)] ( 2 )

Upstream Regulation
Treatments:	LRRK2-IN-1 ( 2 ) , metastatic potential ( 5 )

References
1	Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704 24719451 Curated Info
2	Luerman GC, et al. (2014) Phosphoproteomic evaluation of pharmacological inhibition of leucine-rich repeat kinase 2 reveals significant off-target effects of LRRK-2-IN-1. J Neurochem 128, 561-76 24117733 Curated Info
3	Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21 23312004 Curated Info
4	Rigbolt KT, et al. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal 4, rs3 21406692 Curated Info
5	Wang YT, et al. (2010) An informatics-assisted label-free quantitation strategy that depicts phosphoproteomic profiles in lung cancer cell invasion. J Proteome Res 9, 5582-97 20815410 Curated Info
6	Zhou J (2009) CST Curation Set: 6544; Year: 2009; Biosample/Treatment: cell line, Jurkat/TPA; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PKD Substrate Antibody Cat#: 4381, PTMScan(R) Phospho-PKD Substrate Motif (LXRXXpS/pT) Immunoaffinity Beads Cat#: 1986 Curated Info