Ser19
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Home > Phosphorylation Site Page: > Ser19  -  TH iso3 (human)

Site Information
KGFRRAVsELDAKQA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448542

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 2 ) , mass spectrometry ( 3 , 4 , 7 ) , phospho-antibody ( 1 , 2 , 5 ) , western blotting ( 1 , 2 , 3 , 5 )
Disease tissue studied:
adrenal cancer ( 2 ) , pheochromocytoma ( 2 ) , brain cancer ( 7 ) , glioblastoma ( 7 ) , glioma ( 7 ) , neuroblastoma ( 2 , 4 , 5 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 2 ) , adrenal medulla ( 1 ) , E.coli (bacterial) ( 3 ) , M059K (glial) ( 7 ) , NB10 (neural crest) ( 4 ) , NPC (neural crest) ( 4 ) , PC-12 (chromaffin) ( 2 ) , SH-SY5Y (neural crest) ( 2 , 5 )

Upstream Regulation
Putative in vivo kinases:
CAMK2A (human) ( 1 )
Kinases, in vitro:
CAMK2A (rat) ( 9 , 10 ) , MAPKAPK2 (human) ( 8 , 10 ) , MAPKAPK5 (human) ( 6 , 8 )
Treatments:
ACh ( 1 ) , colforsin ( 5 ) , EGF ( 5 ) , KN-93 ( 1 ) , muscarine ( 5 ) , Shenmai ( 1 ) , U0126 ( 5 )

Downstream Regulation
Effects of modification on TH iso3:
enzymatic activity, induced ( 1 ) , molecular association, regulation ( 3 , 6 , 8 )
Induce interaction with:
14-3-3 gamma (human) ( 3 , 6 ) , 14-3-3 zeta (human) ( 6 ) , 14-3-3 zeta (sheep) ( 8 )

References 

1

Zhang X, et al. (2020) The Inhibition Effects of Shenmai Injection on Acetylcholine-Induced Catecholamine Synthesis and Secretion by Modulating Nicotinic Acetylcholine Receptor Ion Channels in Cultured Bovine Adrenal Medullary Cells. Evid Based Complement Alternat Med 2020, 8514926
33456492   Curated Info

2

Jorge-Finnigan A, et al. (2017) Phosphorylation at serine 31 targets tyrosine hydroxylase to vesicles for transport along microtubules. J Biol Chem 292, 14092-14107
28637871   Curated Info

3

Kleppe R, et al. (2014) Phosphorylation dependence and stoichiometry of the complex formed by tyrosine hydroxylase and 14-3-3γ. Mol Cell Proteomics 13, 2017-30
24947669   Curated Info

4

DeNardo BD, et al. (2013) Quantitative phosphoproteomic analysis identifies activation of the RET and IGF-1R/IR signaling pathways in neuroblastoma. PLoS One 8, e82513
24349301   Curated Info

5

Gordon SL, Bobrovskaya L, Dunkley PR, Dickson PW (2009) Differential regulation of human tyrosine hydroxylase isoforms 1 and 2 in situ: Isoform 2 is not phosphorylated at Ser35. Biochim Biophys Acta 1793, 1860-7
19833152   Curated Info

6

Halskau Ø, et al. (2009) Three-way interaction between 14-3-3 proteins, the N-terminal region of tyrosine hydroxylase, and negatively charged membranes. J Biol Chem 284, 32758-69
19801645   Curated Info

7

Stokes M (2007) CST Curation Set: 2262; Year: 2007; Biosample/Treatment: cell line, M059K/UV; Disease: glioblastoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]Q Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) ATM/ATR Substrate Antibody Cat#: 2851
Curated Info

8

Toska K, et al. (2002) Regulation of tyrosine hydroxylase by stress-activated protein kinases. J Neurochem 83, 775-83
12421349   Curated Info

9

Alterio J, et al. (1998) Human tyrosine hydroxylase isoforms. Inhibition by excess tetrahydropterin and unusual behavior of isoform 3 after camp-dependent protein kinase phosphorylation. J Biol Chem 273, 10196-201
9553069   Curated Info

10

Sutherland C, et al. (1993) Phosphorylation and activation of human tyrosine hydroxylase in vitro by mitogen-activated protein (MAP) kinase and MAP-kinase-activated kinases 1 and 2. Eur J Biochem 217, 715-22
7901013   Curated Info